DNA methylation differences in exposed workers and nearby residents of the Ma Ta phut industrial estate, rayong, Thailand

Marco Peluso, Valentina Bollati, Armelle Munnia, Petcharin Srivatanakul, Adisorn Jedpiyawongse, Suleeporn Sangrajrang, Sara Piro, Marcello Ceppi, Pier Alberto Bertazzi, Paolo Boffetta, Andrea A. Baccarelli

Research output: Contribution to journalArticle

Abstract

Background: Adverse biological effects from airborne pollutants are a primary environmental concern in highly industrialized areas. Recent studies linked air pollution exposures with altered blood Deoxyribo-nucleic acid (DNA) methylation, but effects from industrial sources and underlying biological mechanisms are still largely unexplored. Methods: The Ma Ta Phut industrial estate (MIE) in Rayong, Thailand hosts one of the largest steel, oil refinery and petrochemical complexes in south-eastern Asia. We measured a panel of blood DNA methylation markers previously associated with air pollution exposures, including repeated elements [long interspersed nuclear element-1 (LINE-1) and Alu] and genes [p53, hypermethylated-in-cancer-1 (HIC1), p16 and interleukin-6 (IL-6)], in 67 MIE workers, 65 Ma Ta Phut residents and 45 rural controls. To evaluate the role of DNA damage and oxidation, we correlated DNA methylation measures with bulky DNA and 3-(2-deoxy-β-D-erythro-pentafuranosyl)pyrimido[1,2-α]purin-10(3H)-one deoxyguanosine (M1dG) adducts. Results: In covariate-adjusted models, MIE workers, compared with rural residents, showed lower LINE-1 (74.8% vs 78.0%; P <0.001), p53 (8.0% vs 15.7%; P <0.001) and IL-6 methylation (39.2% vs 45.0%; P = 0.027) and higher HIC1 methylation (22.2% vs 15.3%, P <0.001). For all four markers, Ma Ta Phut residents exhibited methylation levels intermediate between MIE workers and rural controls (LINE-1, 75.7%, P <0.001; p53, 9.0%, P <0.001; IL-6, 39.8%, P = 0.041; HIC1, 17.8%, P = 0.05; all P-values vs rural controls). Bulky DNA adducts showed negative correlation with p53 methylation (P = 0.01). M1dG showed negative correlations with LINE-1 (P = 0.003) and IL-6 methylation (P = 0.05). Conclusions: Our findings indicate that industrial exposures may induce alterations of DNA methylation patterns detectable in blood leucocyte DNA. Correlation of DNA adducts with DNA hypomethylation suggests potential mediation by DNA damage.

Original languageEnglish
Article numberdys129
Pages (from-to)1753-1760
Number of pages8
JournalInternational Journal of Epidemiology
Volume41
Issue number6
DOIs
Publication statusPublished - Dec 2012

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Keywords

  • Air pollution
  • Blood leucocytes
  • DNA damage
  • DNA methylation
  • Ma Ta Phut

ASJC Scopus subject areas

  • Epidemiology

Cite this

Peluso, M., Bollati, V., Munnia, A., Srivatanakul, P., Jedpiyawongse, A., Sangrajrang, S., Piro, S., Ceppi, M., Bertazzi, P. A., Boffetta, P., & Baccarelli, A. A. (2012). DNA methylation differences in exposed workers and nearby residents of the Ma Ta phut industrial estate, rayong, Thailand. International Journal of Epidemiology, 41(6), 1753-1760. [dys129]. https://doi.org/10.1093/ije/dys129