DNA microarray for detection of macrolide resistance genes

Marco Cassone, Marco M. D'Andrea, Francesco Iannelli, Marco R. Oggioni, Gian Maria Rossolini, Gianni Pozzi

Research output: Contribution to journalArticle

Abstract

A DNA microarray was developed to detect bacterial genes conferring resistance to macrolides and related antibiotics. A database containing 65 nonredundant genes selected from publicly available DNA sequences was constructed and used to design 100 oligonucleotide probes that could specifically detect and discriminate all 65 genes. Probes were spotted on a glass slide, and the array was reacted with DNA templates extracted from 20 reference strains of eight different bacterial species (Streptococcus pneumoniae, Streptococcus pyogenes, Enterococcus faecalis, Enterococcus faecium, Staphylococcus aureus, Staphylococcus haemolyticus, Escherichia coli, and Bacteroides fragilis) known to harbor 29 different macrolide resistance genes. Hybridization results showed that probes reacted with, and only with, the expected DNA templates and allowed discovery of three unexpected genes, including msr(SA) in B. fragilis, an efflux gene that has not yet been described for gram-negative bacteria.

Original languageEnglish
Pages (from-to)2038-2041
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume50
Issue number6
DOIs
Publication statusPublished - Jun 2006

ASJC Scopus subject areas

  • Pharmacology (medical)

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    Cassone, M., D'Andrea, M. M., Iannelli, F., Oggioni, M. R., Rossolini, G. M., & Pozzi, G. (2006). DNA microarray for detection of macrolide resistance genes. Antimicrobial Agents and Chemotherapy, 50(6), 2038-2041. https://doi.org/10.1128/AAC.01574-05