DNA mismatch repair protein expression and microsatellite instability in primary mucosal melanomas of the head and neck

C. Marani, E. Alvino, S. Caporali, M. G. Vigili, G. Mancini, S. Rahimi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aims: To examine the expression of DNA mismatch repair (MMR) proteins and the presence of microsatellite instability (MSI) in seven primary mucosal melanomas of the head and neck (MMHN). Methods and results: Haematoxylin and eosin staining and immunohistochemical analysis for routine diagnostic markers and for MMR proteins were performed. Six cases were examined for MSI. Four cases were monomorphous and three cases were pleomorphic type MMHN. Melanocytic markers were positive in all cases. Immunoreactivity for MMR proteins was weak in normal epithelium. The neoplastic tissue in six cases showed positivity for all MMR proteins with different percentages. One case showed weak positivity for hMSH2 and hMSH6 and no immunoreactivity for hMLH1 or hPMS2. Staining intensity was higher in tumour cells than in matched normal mucosa in three cases for hMSH2 and hMLH1 and in two cases for hPMS2. None of the examined cases showed MSI. Conclusions: Expression of hMSH2 and hMLH1 proteins was up-regulated in three cases, whereas in two cases that of hPMS2 was increased. hMSH6 expression was comparable to that of normal cells in all cases. The percentage of positive neoplastic cells and the intensity of staining seemed to be greater in pleomorphic melanomas. Six cases were MMR-proficient and microsatellite stable.

Original languageEnglish
Pages (from-to)780-788
Number of pages9
JournalHistopathology
Volume50
Issue number6
DOIs
Publication statusPublished - May 2007

Fingerprint

Microsatellite Instability
DNA Mismatch Repair
Melanoma
Neck
Head
Staining and Labeling
Proteins
Hematoxylin
Eosine Yellowish-(YS)
Microsatellite Repeats
Mucous Membrane
Epithelium
Neoplasms

Keywords

  • DNA
  • Microsatellite instability
  • Mismatch repair proteins
  • MMR
  • MSI
  • Mucosal melanoma

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Cell Biology

Cite this

DNA mismatch repair protein expression and microsatellite instability in primary mucosal melanomas of the head and neck. / Marani, C.; Alvino, E.; Caporali, S.; Vigili, M. G.; Mancini, G.; Rahimi, S.

In: Histopathology, Vol. 50, No. 6, 05.2007, p. 780-788.

Research output: Contribution to journalArticle

Marani, C. ; Alvino, E. ; Caporali, S. ; Vigili, M. G. ; Mancini, G. ; Rahimi, S. / DNA mismatch repair protein expression and microsatellite instability in primary mucosal melanomas of the head and neck. In: Histopathology. 2007 ; Vol. 50, No. 6. pp. 780-788.
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abstract = "Aims: To examine the expression of DNA mismatch repair (MMR) proteins and the presence of microsatellite instability (MSI) in seven primary mucosal melanomas of the head and neck (MMHN). Methods and results: Haematoxylin and eosin staining and immunohistochemical analysis for routine diagnostic markers and for MMR proteins were performed. Six cases were examined for MSI. Four cases were monomorphous and three cases were pleomorphic type MMHN. Melanocytic markers were positive in all cases. Immunoreactivity for MMR proteins was weak in normal epithelium. The neoplastic tissue in six cases showed positivity for all MMR proteins with different percentages. One case showed weak positivity for hMSH2 and hMSH6 and no immunoreactivity for hMLH1 or hPMS2. Staining intensity was higher in tumour cells than in matched normal mucosa in three cases for hMSH2 and hMLH1 and in two cases for hPMS2. None of the examined cases showed MSI. Conclusions: Expression of hMSH2 and hMLH1 proteins was up-regulated in three cases, whereas in two cases that of hPMS2 was increased. hMSH6 expression was comparable to that of normal cells in all cases. The percentage of positive neoplastic cells and the intensity of staining seemed to be greater in pleomorphic melanomas. Six cases were MMR-proficient and microsatellite stable.",
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AU - Rahimi, S.

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N2 - Aims: To examine the expression of DNA mismatch repair (MMR) proteins and the presence of microsatellite instability (MSI) in seven primary mucosal melanomas of the head and neck (MMHN). Methods and results: Haematoxylin and eosin staining and immunohistochemical analysis for routine diagnostic markers and for MMR proteins were performed. Six cases were examined for MSI. Four cases were monomorphous and three cases were pleomorphic type MMHN. Melanocytic markers were positive in all cases. Immunoreactivity for MMR proteins was weak in normal epithelium. The neoplastic tissue in six cases showed positivity for all MMR proteins with different percentages. One case showed weak positivity for hMSH2 and hMSH6 and no immunoreactivity for hMLH1 or hPMS2. Staining intensity was higher in tumour cells than in matched normal mucosa in three cases for hMSH2 and hMLH1 and in two cases for hPMS2. None of the examined cases showed MSI. Conclusions: Expression of hMSH2 and hMLH1 proteins was up-regulated in three cases, whereas in two cases that of hPMS2 was increased. hMSH6 expression was comparable to that of normal cells in all cases. The percentage of positive neoplastic cells and the intensity of staining seemed to be greater in pleomorphic melanomas. Six cases were MMR-proficient and microsatellite stable.

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