DNA-nuclease activity of the single-chain ribosome-inactivating proteins dianthin 30, saporin 6 and gelonin

L. Roncuzzi, A. Gasperi-Campani

Research output: Contribution to journalArticle

Abstract

The single-chain ribosome-inactivating proteins (sc-RIPs) from plant origin are antiviral and antiproliferative agents employed in the preparation of immunotoxins, Similarly to the A-chains of ricin, sc-RIPs act as rRNA N-glycosidases. We demonstrate here that dianthin 30, saporin 6 and gelonin exert a specific nuclease activity on supercoiled DNA. Four specific sites of cleavage introduced by dianthin 30 and by saporin 6 and two specific sites of cleavage introduced by gelonin have been identified and mapped in pBR322.

Original languageEnglish
Pages (from-to)16-20
Number of pages5
JournalFEBS Letters
Volume392
Issue number1
DOIs
Publication statusPublished - Aug 19 1996

Keywords

  • Dianthin 30
  • DNAse activity
  • Gelonin
  • Ribosome-inactivating protein
  • Saporin 6
  • Supercoiled DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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