Abstract
The single-chain ribosome-inactivating proteins (sc-RIPs) from plant origin are antiviral and antiproliferative agents employed in the preparation of immunotoxins, Similarly to the A-chains of ricin, sc-RIPs act as rRNA N-glycosidases. We demonstrate here that dianthin 30, saporin 6 and gelonin exert a specific nuclease activity on supercoiled DNA. Four specific sites of cleavage introduced by dianthin 30 and by saporin 6 and two specific sites of cleavage introduced by gelonin have been identified and mapped in pBR322.
Original language | English |
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Pages (from-to) | 16-20 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 392 |
Issue number | 1 |
DOIs | |
Publication status | Published - Aug 19 1996 |
Keywords
- Dianthin 30
- DNAse activity
- Gelonin
- Ribosome-inactivating protein
- Saporin 6
- Supercoiled DNA
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology