DNA ploidy analysis within selected regions of colorectal adenomas containing carcinoma

M. Risio, E. Geido, A. Divinci, A. Rapallo, N. Pujic, F. P. Rossini, W. Giaretti

Research output: Contribution to journalArticle

Abstract

In order to better understand the relationship of DNA ploidy, dysplasia, early cancer, and colorectal tumor progression, 11 colorectal adenomas containing carcinoma invading the submucosa were investigated using DNA flow cytometry. Multiple frozen samples were taken from the selected sectors corresponding to adenoma tissue with low-grade dysplasia, high grade dysplasia and early cancer. Sampling accuracy was performed under histologic examination by multiple cryostatic sections. Data were compared with previously reported results in non-cancerous adenomas and advanced carcinomas. Incidence of DNA aneuploidy among the dysplastic regions of the adenomas containing carcinomas resulted higher than that observed in non- cancerous adenomas (p=0.02). Furthermore, among the DNA aneuploid populations, the frequency of clones with high DNA Index (D1>1.3) was slightly higher in adenomas with cancer than in adenomas without cancer (p=0.07). We suggest that differences may exist in DNA aneuploidy evolution between these two types of lesions. In early cancer, the near-diploid clones were 57% with respect to 18% (p=0.01) in advanced cancer since in this latter case the majority of the DNA abnormal clones were in the near-hypertriploid region (82%). Thus, the acquisition of the invasive phenotype appears to be linked with the expansion and stabilization of high DNA aneuploid clones. Further analysis on a larger number of cases of adenomas containing carcinoma are necessary to validate these interpretations.

Original languageEnglish
Pages (from-to)941-947
Number of pages7
JournalInternational Journal of Oncology
Volume3
Issue number5
Publication statusPublished - 1993

Fingerprint

Ploidies
Adenoma
Carcinoma
DNA
Aneuploidy
Clone Cells
Neoplasms
Diploidy
Colorectal Neoplasms
Flow Cytometry
Phenotype
Incidence
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Risio, M., Geido, E., Divinci, A., Rapallo, A., Pujic, N., Rossini, F. P., & Giaretti, W. (1993). DNA ploidy analysis within selected regions of colorectal adenomas containing carcinoma. International Journal of Oncology, 3(5), 941-947.

DNA ploidy analysis within selected regions of colorectal adenomas containing carcinoma. / Risio, M.; Geido, E.; Divinci, A.; Rapallo, A.; Pujic, N.; Rossini, F. P.; Giaretti, W.

In: International Journal of Oncology, Vol. 3, No. 5, 1993, p. 941-947.

Research output: Contribution to journalArticle

Risio, M, Geido, E, Divinci, A, Rapallo, A, Pujic, N, Rossini, FP & Giaretti, W 1993, 'DNA ploidy analysis within selected regions of colorectal adenomas containing carcinoma', International Journal of Oncology, vol. 3, no. 5, pp. 941-947.
Risio M, Geido E, Divinci A, Rapallo A, Pujic N, Rossini FP et al. DNA ploidy analysis within selected regions of colorectal adenomas containing carcinoma. International Journal of Oncology. 1993;3(5):941-947.
Risio, M. ; Geido, E. ; Divinci, A. ; Rapallo, A. ; Pujic, N. ; Rossini, F. P. ; Giaretti, W. / DNA ploidy analysis within selected regions of colorectal adenomas containing carcinoma. In: International Journal of Oncology. 1993 ; Vol. 3, No. 5. pp. 941-947.
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