Abstract
Background/Aims: The purpose of this study was to define the prognostic role of DNA ploidy, proliferative index and EGF-R status in resected gastric cancer. Materials and Methods: Ten clinico-pathological parameters and three biological factors obtained from flow cytometry and immunohisto-chemistry were evaluated in a series of 130 gastric cancer patients who received surgical treatment, including 28 stage IV cases (21.6%), using paraffin-embedded and fresh specimens in 77.7% and 22.3% of the cases, respectively. These variables were first analyzed and tested for correlation within the whole series and then weighted against survival in 117 applicable cases through univariate and multivariate analyses. Results: Aneuploidy was significantly related to higher proliferative activity, EGF-R expression and deeper stomach wall infiltration. Higher proliferative activity was significantly related to deeper stomach wall infiltration and larger tumor diameter. The latter showed a significant relationship to EGF-R expression. Univariate analysis showed the significant variables for survival to be DNA ploidy, pT, pN, M, stage, histological type according to Lauren and tumor diameter. Multivariate analysis calculated on, these significant variables using the Cox multiple stepwise regression, model detected three factors which independently influence survival: pathological stage (p <0.00001), histological type according to Lauren (p <0.002) and DNA ploidy (p <0.03). Conclusions: DNA ploidy was shown to be a significant prognostic parameter in resected gastric cancer after pathological stage and histological type according to Lauren. The prognostic roles of proliferative activity and EGF-R status require further investigation.
| Original language | English |
|---|---|
| Pages (from-to) | 826-837 |
| Number of pages | 12 |
| Journal | Hepato-Gastroenterology |
| Volume | 44 |
| Issue number | 15 |
| Publication status | Published - 1997 |
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Keywords
- DNA ploidy
- EGF-R status
- Gastric cancer
- Proliferative index
ASJC Scopus subject areas
- Gastroenterology
Cite this
DNA ploidy, proliferative index and EGF-R status in 130 cases of resected gastric cancer - a multivariate analysis. / Santoro, Eugenio; Carboni, Manlio; Catarci, Marco; Carlini, Massimo; Carboni, Fabio; Zupi, Gabriella; Vecchione, Aldo; D'Agnano, Igea; Giannarelli, Diana; Santoro, Roberto; Garofalo, Alfredo.
In: Hepato-Gastroenterology, Vol. 44, No. 15, 1997, p. 826-837.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA ploidy, proliferative index and EGF-R status in 130 cases of resected gastric cancer - a multivariate analysis
AU - Santoro, Eugenio
AU - Carboni, Manlio
AU - Catarci, Marco
AU - Carlini, Massimo
AU - Carboni, Fabio
AU - Zupi, Gabriella
AU - Vecchione, Aldo
AU - D'Agnano, Igea
AU - Giannarelli, Diana
AU - Santoro, Roberto
AU - Garofalo, Alfredo
PY - 1997
Y1 - 1997
N2 - Background/Aims: The purpose of this study was to define the prognostic role of DNA ploidy, proliferative index and EGF-R status in resected gastric cancer. Materials and Methods: Ten clinico-pathological parameters and three biological factors obtained from flow cytometry and immunohisto-chemistry were evaluated in a series of 130 gastric cancer patients who received surgical treatment, including 28 stage IV cases (21.6%), using paraffin-embedded and fresh specimens in 77.7% and 22.3% of the cases, respectively. These variables were first analyzed and tested for correlation within the whole series and then weighted against survival in 117 applicable cases through univariate and multivariate analyses. Results: Aneuploidy was significantly related to higher proliferative activity, EGF-R expression and deeper stomach wall infiltration. Higher proliferative activity was significantly related to deeper stomach wall infiltration and larger tumor diameter. The latter showed a significant relationship to EGF-R expression. Univariate analysis showed the significant variables for survival to be DNA ploidy, pT, pN, M, stage, histological type according to Lauren and tumor diameter. Multivariate analysis calculated on, these significant variables using the Cox multiple stepwise regression, model detected three factors which independently influence survival: pathological stage (p <0.00001), histological type according to Lauren (p <0.002) and DNA ploidy (p <0.03). Conclusions: DNA ploidy was shown to be a significant prognostic parameter in resected gastric cancer after pathological stage and histological type according to Lauren. The prognostic roles of proliferative activity and EGF-R status require further investigation.
AB - Background/Aims: The purpose of this study was to define the prognostic role of DNA ploidy, proliferative index and EGF-R status in resected gastric cancer. Materials and Methods: Ten clinico-pathological parameters and three biological factors obtained from flow cytometry and immunohisto-chemistry were evaluated in a series of 130 gastric cancer patients who received surgical treatment, including 28 stage IV cases (21.6%), using paraffin-embedded and fresh specimens in 77.7% and 22.3% of the cases, respectively. These variables were first analyzed and tested for correlation within the whole series and then weighted against survival in 117 applicable cases through univariate and multivariate analyses. Results: Aneuploidy was significantly related to higher proliferative activity, EGF-R expression and deeper stomach wall infiltration. Higher proliferative activity was significantly related to deeper stomach wall infiltration and larger tumor diameter. The latter showed a significant relationship to EGF-R expression. Univariate analysis showed the significant variables for survival to be DNA ploidy, pT, pN, M, stage, histological type according to Lauren and tumor diameter. Multivariate analysis calculated on, these significant variables using the Cox multiple stepwise regression, model detected three factors which independently influence survival: pathological stage (p <0.00001), histological type according to Lauren (p <0.002) and DNA ploidy (p <0.03). Conclusions: DNA ploidy was shown to be a significant prognostic parameter in resected gastric cancer after pathological stage and histological type according to Lauren. The prognostic roles of proliferative activity and EGF-R status require further investigation.
KW - DNA ploidy
KW - EGF-R status
KW - Gastric cancer
KW - Proliferative index
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UR - http://www.scopus.com/inward/citedby.url?scp=8544237779&partnerID=8YFLogxK
M3 - Article
VL - 44
SP - 826
EP - 837
JO - Acta hepato-splenologica
JF - Acta hepato-splenologica
SN - 0172-6390
IS - 15
ER -