DNA ploidy, proliferative index and EGF-R status in 130 cases of resected gastric cancer - a multivariate analysis

Eugenio Santoro, Manlio Carboni, Marco Catarci, Massimo Carlini, Fabio Carboni, Gabriella Zupi, Aldo Vecchione, Igea D'Agnano, Diana Giannarelli, Roberto Santoro, Alfredo Garofalo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background/Aims: The purpose of this study was to define the prognostic role of DNA ploidy, proliferative index and EGF-R status in resected gastric cancer. Materials and Methods: Ten clinico-pathological parameters and three biological factors obtained from flow cytometry and immunohisto-chemistry were evaluated in a series of 130 gastric cancer patients who received surgical treatment, including 28 stage IV cases (21.6%), using paraffin-embedded and fresh specimens in 77.7% and 22.3% of the cases, respectively. These variables were first analyzed and tested for correlation within the whole series and then weighted against survival in 117 applicable cases through univariate and multivariate analyses. Results: Aneuploidy was significantly related to higher proliferative activity, EGF-R expression and deeper stomach wall infiltration. Higher proliferative activity was significantly related to deeper stomach wall infiltration and larger tumor diameter. The latter showed a significant relationship to EGF-R expression. Univariate analysis showed the significant variables for survival to be DNA ploidy, pT, pN, M, stage, histological type according to Lauren and tumor diameter. Multivariate analysis calculated on, these significant variables using the Cox multiple stepwise regression, model detected three factors which independently influence survival: pathological stage (p <0.00001), histological type according to Lauren (p <0.002) and DNA ploidy (p <0.03). Conclusions: DNA ploidy was shown to be a significant prognostic parameter in resected gastric cancer after pathological stage and histological type according to Lauren. The prognostic roles of proliferative activity and EGF-R status require further investigation.

Original languageEnglish
Pages (from-to)826-837
Number of pages12
JournalHepato-Gastroenterology
Volume44
Issue number15
Publication statusPublished - 1997

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Ploidies
Epidermal Growth Factor
Stomach Neoplasms
Multivariate Analysis
DNA
Survival
Stomach
Biological Factors
Aneuploidy
Paraffin
Neoplasms
Flow Cytometry
Therapeutics

Keywords

  • DNA ploidy
  • EGF-R status
  • Gastric cancer
  • Proliferative index

ASJC Scopus subject areas

  • Gastroenterology

Cite this

DNA ploidy, proliferative index and EGF-R status in 130 cases of resected gastric cancer - a multivariate analysis. / Santoro, Eugenio; Carboni, Manlio; Catarci, Marco; Carlini, Massimo; Carboni, Fabio; Zupi, Gabriella; Vecchione, Aldo; D'Agnano, Igea; Giannarelli, Diana; Santoro, Roberto; Garofalo, Alfredo.

In: Hepato-Gastroenterology, Vol. 44, No. 15, 1997, p. 826-837.

Research output: Contribution to journalArticle

Santoro, E, Carboni, M, Catarci, M, Carlini, M, Carboni, F, Zupi, G, Vecchione, A, D'Agnano, I, Giannarelli, D, Santoro, R & Garofalo, A 1997, 'DNA ploidy, proliferative index and EGF-R status in 130 cases of resected gastric cancer - a multivariate analysis', Hepato-Gastroenterology, vol. 44, no. 15, pp. 826-837.
Santoro E, Carboni M, Catarci M, Carlini M, Carboni F, Zupi G et al. DNA ploidy, proliferative index and EGF-R status in 130 cases of resected gastric cancer - a multivariate analysis. Hepato-Gastroenterology. 1997;44(15):826-837.
Santoro, Eugenio ; Carboni, Manlio ; Catarci, Marco ; Carlini, Massimo ; Carboni, Fabio ; Zupi, Gabriella ; Vecchione, Aldo ; D'Agnano, Igea ; Giannarelli, Diana ; Santoro, Roberto ; Garofalo, Alfredo. / DNA ploidy, proliferative index and EGF-R status in 130 cases of resected gastric cancer - a multivariate analysis. In: Hepato-Gastroenterology. 1997 ; Vol. 44, No. 15. pp. 826-837.
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AU - Carlini, Massimo

AU - Carboni, Fabio

AU - Zupi, Gabriella

AU - Vecchione, Aldo

AU - D'Agnano, Igea

AU - Giannarelli, Diana

AU - Santoro, Roberto

AU - Garofalo, Alfredo

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