DNA repair after gamma radiation and superoxide dismutase activity in lymphocytes from subjects of far advanced age

F. Licastro, C. Franceschi, M. Chiricolo, M. G. Battelli, P. Tabacchi, M. Cenci, F. Barboni, D. Pallenzona

Research output: Contribution to journalArticlepeer-review

Abstract

DNA repair after gamma radiation was studied in purified T lymphocytes from young and aged subjects. Two different assays were employed. In the first, T lymphocytes were stimulated with phytohemagglutinin (PHA) for 72 h and then treated with hydroxyurea, irradiated with 30 K rads and pulsed with [3H]thymidine (TdR) for 4 h. In the second, T lymphocytes were first irradiated with graded doses of gamma rays (200-800 rads) and then stimulated with PHA, cultured for 72 h and pulsed with 3H-TdR for the last 6 h of culture. T lymphocytes from aged subjects showed a lack of DNA repair synthesis in the first assay whereas only minor differences were found in the second assay between the two groups, i.e., a certain degree of radioresistance in aged lymphocytes. Lymphocyte superoxide dismutase activity showed great individual variations in both groups and a slight increase in old subjects.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalCarcinogenesis
Volume3
Issue number1
Publication statusPublished - 1982

ASJC Scopus subject areas

  • Cancer Research
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience

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