DNA sequences complementary to human 7 SK RNA show structural similarities to the short mobile elements of the mammalian genome

Shona Murphy, F. Altruda, Elisabetta Ullu, M. Tripodi, L. Silengo, Marialuisa Melli

Research output: Contribution to journalArticle

Abstract

A complementary DNA clone of 7 SK RNA from HeLa cells was used to study the genomic organization of 7 SK sequences in the human genome. Genomic hybridizations and genomic clones show that 7 SK is homologous to a family of disperse repeated sequences most of which lack the 3′ end of the 7 SK RNA sequence. Only few of the genomic K sequences are homologous to both 3′ and 5′ 7 SK probes and presumably include the gene(s) for 7 SK RNA. The sequence of four genomic 7 SK clones confirms that they are in most cases pseudogenes. Although Alu sequences are frequently found near the 3′ and 5′ end of K DNA, the sequences immediately flanking the pseudogenes are different in all clones studied. However, direct repeats were found flanking directly the K DNA or the K-Alu unit, suggesting that the K sequences alone or in conjunction with Alu DNA might constitute a mobile element.

Original languageEnglish
Pages (from-to)575-590
Number of pages16
JournalJournal of Molecular Biology
Volume177
Issue number4
DOIs
Publication statusPublished - Aug 25 1984

ASJC Scopus subject areas

  • Virology

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