DNA vaccines against HPV-16 E7-expressing tumour cells

Federico De Marco, Sophie Hallez, Jean Marc Brulet, Fabienne Gesché, Pasqualina Marzano, Silvio Flamini, Maria Luisa Marcante, Aldo Venuti

Research output: Contribution to journalArticle

Abstract

Background: Genetic immunisation induces the endogenous production of the encoded antigens, which favours their presentation by MHC class I molecules. The E7 protein from "high risk" Human Papillomavirus (HPV) is constitutively expressed in cervical cancer and represents a target for immunotherapy. Materials and Methods: Several E7-encoding DNA vaccines were constructed including unmodified E7 and E7 fused to ubiquitin or to the Invariant chain in order to increase the presentation of E7-derived peptides by MHC class I or II molecules, respectively. These vaccines were administered i.m. to C57BL/6 mice that were subsequently challenged with E7-positive tumour cell lines expressing different levels of MHC class I molecules. Results: The E7-Ii fusion sequence protected a number of animals from tumour challenging. No differences were associated with the MHC class I status of the challenging cell lines. Conclusion: Engineering the intracellular pathway for antigen presentation is able to produce a valid therapeutic response even against tumours with down-regulated MHC class I.

Original languageEnglish
Pages (from-to)1449-1454
Number of pages6
JournalAnticancer Research
Volume23
Issue number2 B
Publication statusPublished - Mar 2003

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Keywords

  • E7 protein
  • Genetic immunisation
  • HPV-16
  • MHC expression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

De Marco, F., Hallez, S., Brulet, J. M., Gesché, F., Marzano, P., Flamini, S., Marcante, M. L., & Venuti, A. (2003). DNA vaccines against HPV-16 E7-expressing tumour cells. Anticancer Research, 23(2 B), 1449-1454.