Does Fluoroscopy Induce DNA Oxidative Damage in Patients Undergoing Catheter Ablation?

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Abstract

As no studies before now have thoroughly investigated the risk associated with the exposure to low-dose ionizing radiations in patients undergoing catheter ablation (CA), we aimed to evaluate the oxidative and DNA damage in 59 CA patients (10 of whom received N-acetylcysteine (NAC) before the procedure). A burst of oxidized/reduced glutathione ratio was observed 3 hours after procedure that was diminished by NAC administration. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) concentrations, index of oxidative DNA damage, showed a peak 24 hours after CA. A positive correlation between 8-OHdG peak and fluoroscopy time and a negative correlation among 8-OHdG decrease (from the peak to 48 hours after CA) and all procedure parameters were found. Furthermore, DNA tail percentages (which reflect the extent and the number of DNA strand breaks) positively correlated with 8-OHdG concentrations. This study evaluates for the first time the kinetic of oxidative damage in patients undergoing CA procedure. Our findings raise the question of whether 8-OHdG can be used as a circulating biomarker of DNA oxidative damage induced by low-dose ionizing radiations in different clinical settings. Antioxid. Redox Signal. 00, 000-000.

Original languageEnglish
JournalAntioxidants and Redox Signaling
DOIs
Publication statusE-pub ahead of print - Nov 27 2017

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Catheter Ablation
Catheters
Fluoroscopy
Ablation
DNA Damage
DNA
Ionizing radiation
Acetylcysteine
Ionizing Radiation
Dosimetry
DNA Breaks
Glutathione Disulfide
Biomarkers
Oxidation-Reduction
Glutathione
Kinetics

Keywords

  • Journal Article

Cite this

@article{d25c570ecb5e49e5ab111e9f79de09da,
title = "Does Fluoroscopy Induce DNA Oxidative Damage in Patients Undergoing Catheter Ablation?",
abstract = "As no studies before now have thoroughly investigated the risk associated with the exposure to low-dose ionizing radiations in patients undergoing catheter ablation (CA), we aimed to evaluate the oxidative and DNA damage in 59 CA patients (10 of whom received N-acetylcysteine (NAC) before the procedure). A burst of oxidized/reduced glutathione ratio was observed 3 hours after procedure that was diminished by NAC administration. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) concentrations, index of oxidative DNA damage, showed a peak 24 hours after CA. A positive correlation between 8-OHdG peak and fluoroscopy time and a negative correlation among 8-OHdG decrease (from the peak to 48 hours after CA) and all procedure parameters were found. Furthermore, DNA tail percentages (which reflect the extent and the number of DNA strand breaks) positively correlated with 8-OHdG concentrations. This study evaluates for the first time the kinetic of oxidative damage in patients undergoing CA procedure. Our findings raise the question of whether 8-OHdG can be used as a circulating biomarker of DNA oxidative damage induced by low-dose ionizing radiations in different clinical settings. Antioxid. Redox Signal. 00, 000-000.",
keywords = "Journal Article",
author = "Linda Turnu and Benedetta Porro and Valentina Alfieri and {Di Minno}, Alessandro and Eleonora Russo and Simone Barbieri and Alice Bonomi and {Dello Russo}, Antonio and Claudio Tondo and Yuri D'Alessandra and Viviana Cavalca and Elena Tremoli and Colombo, {Gualtiero I} and Michela Casella",
year = "2017",
month = "11",
day = "27",
doi = "10.1089/ars.2017.7334",
language = "English",
journal = "Antioxidants and Redox Signaling",
issn = "1523-0864",
publisher = "Mary Ann Liebert Inc.",

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TY - JOUR

T1 - Does Fluoroscopy Induce DNA Oxidative Damage in Patients Undergoing Catheter Ablation?

AU - Turnu, Linda

AU - Porro, Benedetta

AU - Alfieri, Valentina

AU - Di Minno, Alessandro

AU - Russo, Eleonora

AU - Barbieri, Simone

AU - Bonomi, Alice

AU - Dello Russo, Antonio

AU - Tondo, Claudio

AU - D'Alessandra, Yuri

AU - Cavalca, Viviana

AU - Tremoli, Elena

AU - Colombo, Gualtiero I

AU - Casella, Michela

PY - 2017/11/27

Y1 - 2017/11/27

N2 - As no studies before now have thoroughly investigated the risk associated with the exposure to low-dose ionizing radiations in patients undergoing catheter ablation (CA), we aimed to evaluate the oxidative and DNA damage in 59 CA patients (10 of whom received N-acetylcysteine (NAC) before the procedure). A burst of oxidized/reduced glutathione ratio was observed 3 hours after procedure that was diminished by NAC administration. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) concentrations, index of oxidative DNA damage, showed a peak 24 hours after CA. A positive correlation between 8-OHdG peak and fluoroscopy time and a negative correlation among 8-OHdG decrease (from the peak to 48 hours after CA) and all procedure parameters were found. Furthermore, DNA tail percentages (which reflect the extent and the number of DNA strand breaks) positively correlated with 8-OHdG concentrations. This study evaluates for the first time the kinetic of oxidative damage in patients undergoing CA procedure. Our findings raise the question of whether 8-OHdG can be used as a circulating biomarker of DNA oxidative damage induced by low-dose ionizing radiations in different clinical settings. Antioxid. Redox Signal. 00, 000-000.

AB - As no studies before now have thoroughly investigated the risk associated with the exposure to low-dose ionizing radiations in patients undergoing catheter ablation (CA), we aimed to evaluate the oxidative and DNA damage in 59 CA patients (10 of whom received N-acetylcysteine (NAC) before the procedure). A burst of oxidized/reduced glutathione ratio was observed 3 hours after procedure that was diminished by NAC administration. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) concentrations, index of oxidative DNA damage, showed a peak 24 hours after CA. A positive correlation between 8-OHdG peak and fluoroscopy time and a negative correlation among 8-OHdG decrease (from the peak to 48 hours after CA) and all procedure parameters were found. Furthermore, DNA tail percentages (which reflect the extent and the number of DNA strand breaks) positively correlated with 8-OHdG concentrations. This study evaluates for the first time the kinetic of oxidative damage in patients undergoing CA procedure. Our findings raise the question of whether 8-OHdG can be used as a circulating biomarker of DNA oxidative damage induced by low-dose ionizing radiations in different clinical settings. Antioxid. Redox Signal. 00, 000-000.

KW - Journal Article

U2 - 10.1089/ars.2017.7334

DO - 10.1089/ars.2017.7334

M3 - Article

JO - Antioxidants and Redox Signaling

JF - Antioxidants and Redox Signaling

SN - 1523-0864

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