Does pilocarpine-induced epilepsy in adult rats require status epilepticus?

Graciela Navarro Mora, Placido Bramanti, Francesco Osculati, Asmaa Chakir, Elena Nicolato, Pasquina Marzola, Andrea Sbarbati, Paolo Francesco Fabene

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Pilocarpine-induced seizures in rats provide a widely animal model of temporal lobe epilepsy. Some evidences reported in the literature suggest that at least 1 h of status epilepticus (SE) is required to produce subsequent chronic phase, due to the SE-related acute neuronal damage. However, recent data seems to indicate that neuro-inflammation plays a crucial role in epileptogenesis, modulating secondarily a neuronal insult. For this reason, we decided to test the following hypotheses: a) whether pilocarpine-injected rats that did not develop SE can exhibit long-term chronic spontaneous recurrent seizures (SRS) and b) whether acute neurodegeneration is mandatory to obtain chronic epilepsy. Therefore, we compared animals injected with the same dose of pilocarpine that developed or did not SE, and saline treated rats. We used telemetric acquisition of EEG as long-term monitoring system to evaluate the occurrence of seizures in non-SE pilocarpineinjected animals. Furthermore, histology and MRI analysis were applied in order to detect neuronal injury and neuropathological signs. Our observations indicate that non-SE rats exhibit SRS almost 8 (+/22) months after pilocarpine-injection, independently to the absence of initial acute neuronal injury. This is the first time reported that pilocarpine injected rats without developing SE, can experience SRS after a long latency period resembling human pathology. Thus, we strongly emphasize the important meaning of including these animals to model human epileptogenesis in pilocarpine induced epilepsy.

Original languageEnglish
Article numbere5759
JournalPLoS One
Volume4
Issue number6
DOIs
Publication statusPublished - Jun 2 2009

Fingerprint

pilocarpine
Pilocarpine
epilepsy
Status Epilepticus
seizures
Rats
Epilepsy
Seizures
rats
Animals
Animal Models
animals
Histology
Temporal Lobe Epilepsy
Wounds and Injuries
Pathology
Electroencephalography
Magnetic resonance imaging
histology
inflammation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Navarro Mora, G., Bramanti, P., Osculati, F., Chakir, A., Nicolato, E., Marzola, P., ... Fabene, P. F. (2009). Does pilocarpine-induced epilepsy in adult rats require status epilepticus? PLoS One, 4(6), [e5759]. https://doi.org/10.1371/journal.pone.0005759

Does pilocarpine-induced epilepsy in adult rats require status epilepticus? / Navarro Mora, Graciela; Bramanti, Placido; Osculati, Francesco; Chakir, Asmaa; Nicolato, Elena; Marzola, Pasquina; Sbarbati, Andrea; Fabene, Paolo Francesco.

In: PLoS One, Vol. 4, No. 6, e5759, 02.06.2009.

Research output: Contribution to journalArticle

Navarro Mora, G, Bramanti, P, Osculati, F, Chakir, A, Nicolato, E, Marzola, P, Sbarbati, A & Fabene, PF 2009, 'Does pilocarpine-induced epilepsy in adult rats require status epilepticus?', PLoS One, vol. 4, no. 6, e5759. https://doi.org/10.1371/journal.pone.0005759
Navarro Mora G, Bramanti P, Osculati F, Chakir A, Nicolato E, Marzola P et al. Does pilocarpine-induced epilepsy in adult rats require status epilepticus? PLoS One. 2009 Jun 2;4(6). e5759. https://doi.org/10.1371/journal.pone.0005759
Navarro Mora, Graciela ; Bramanti, Placido ; Osculati, Francesco ; Chakir, Asmaa ; Nicolato, Elena ; Marzola, Pasquina ; Sbarbati, Andrea ; Fabene, Paolo Francesco. / Does pilocarpine-induced epilepsy in adult rats require status epilepticus?. In: PLoS One. 2009 ; Vol. 4, No. 6.
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