Does the Presence of an Epiretinal Membrane Alter the Cleavage Plane during Internal Limiting Membrane Peeling?

Nihal Kenawy, David Wong, Theodore Stappler, Mario R. Romano, Ronald A. Das, Gillian Hebbar, Wendy Prime, Heinrich Heimann, Syed K. Gibran, Carl M. Sheridan, Yin Him Cheung, Paul S. Hiscott

Research output: Contribution to journalArticle

Abstract

Purpose: To determine whether the presence of a clinically and/or microscopically detectable epiretinal membrane (ERM) alters the cleavage plane during internal limiting membrane (ILM) peeling. Design: Retrospective, observational, immunohistochemical study of ILM specimens using archival formalin-fixed, paraffin-embedded tissue. Participants: Fifty-one patients who had had ILM excision. Methods: Fifty-one ILM specimens peeled during vitrectomy for various etiologies were examined by light microscopy. The removal of ILM was assisted using Trypan blue (n = 30), indocyanine green (n = 7), or brilliant blue G (n = 14). Monoclonal antibodies to glial fibrillary acidic protein and to neurofilament protein were used to detect glial or neuronal cells respectively on the vitreous or retinal surfaces of the ILM. Specimens were divided into 2 groups: ILM peeled for full-thickness macular hole (MH; n = 31) and ILM peeled after removal of clinically detectable ERM (n = 20). Main Outcome Measures: Primary outcome measure was the localization of immunohistochemical markers to neuronal or glial cells on the vitreous or retinal surfaces of ILM. The secondary outcome measure was the correlation of the results of the primary measure with the dyes used to facilitate ILM peeling. Results: Glial and/or neuronal cells were detected on the retinal surface of the ILM in 10 of 31 (32%) of the MH ILM specimens and in 13 of 20 (65%) of the ILM peeled after ERM excision; the difference was significant (P = 0.02). There was no association between the presence of neuronal and glial cells with the type of dye used (P = 0.2). Of the 23 ILM specimens with cells attached to the retinal surface, 21 (91%) were associated with clinical and/or histologic evidence of ERM and 2 (9%) were not. The correlation between the presence of cells on the vitreous and the retinal surfaces of ILM was high (P

Original languageEnglish
JournalOphthalmology
Volume117
Issue number2
DOIs
Publication statusPublished - Feb 2010

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Epiretinal Membrane
Membranes
Neuroglia
Outcome Assessment (Health Care)
Coloring Agents
Neurofilament Proteins
Retinal Perforations
Indocyanine Green
Trypan Blue
Glial Fibrillary Acidic Protein
Vitrectomy

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Kenawy, N., Wong, D., Stappler, T., Romano, M. R., Das, R. A., Hebbar, G., ... Hiscott, P. S. (2010). Does the Presence of an Epiretinal Membrane Alter the Cleavage Plane during Internal Limiting Membrane Peeling? Ophthalmology, 117(2). https://doi.org/10.1016/j.ophtha.2009.07.024

Does the Presence of an Epiretinal Membrane Alter the Cleavage Plane during Internal Limiting Membrane Peeling? / Kenawy, Nihal; Wong, David; Stappler, Theodore; Romano, Mario R.; Das, Ronald A.; Hebbar, Gillian; Prime, Wendy; Heimann, Heinrich; Gibran, Syed K.; Sheridan, Carl M.; Cheung, Yin Him; Hiscott, Paul S.

In: Ophthalmology, Vol. 117, No. 2, 02.2010.

Research output: Contribution to journalArticle

Kenawy, N, Wong, D, Stappler, T, Romano, MR, Das, RA, Hebbar, G, Prime, W, Heimann, H, Gibran, SK, Sheridan, CM, Cheung, YH & Hiscott, PS 2010, 'Does the Presence of an Epiretinal Membrane Alter the Cleavage Plane during Internal Limiting Membrane Peeling?', Ophthalmology, vol. 117, no. 2. https://doi.org/10.1016/j.ophtha.2009.07.024
Kenawy, Nihal ; Wong, David ; Stappler, Theodore ; Romano, Mario R. ; Das, Ronald A. ; Hebbar, Gillian ; Prime, Wendy ; Heimann, Heinrich ; Gibran, Syed K. ; Sheridan, Carl M. ; Cheung, Yin Him ; Hiscott, Paul S. / Does the Presence of an Epiretinal Membrane Alter the Cleavage Plane during Internal Limiting Membrane Peeling?. In: Ophthalmology. 2010 ; Vol. 117, No. 2.
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abstract = "Purpose: To determine whether the presence of a clinically and/or microscopically detectable epiretinal membrane (ERM) alters the cleavage plane during internal limiting membrane (ILM) peeling. Design: Retrospective, observational, immunohistochemical study of ILM specimens using archival formalin-fixed, paraffin-embedded tissue. Participants: Fifty-one patients who had had ILM excision. Methods: Fifty-one ILM specimens peeled during vitrectomy for various etiologies were examined by light microscopy. The removal of ILM was assisted using Trypan blue (n = 30), indocyanine green (n = 7), or brilliant blue G (n = 14). Monoclonal antibodies to glial fibrillary acidic protein and to neurofilament protein were used to detect glial or neuronal cells respectively on the vitreous or retinal surfaces of the ILM. Specimens were divided into 2 groups: ILM peeled for full-thickness macular hole (MH; n = 31) and ILM peeled after removal of clinically detectable ERM (n = 20). Main Outcome Measures: Primary outcome measure was the localization of immunohistochemical markers to neuronal or glial cells on the vitreous or retinal surfaces of ILM. The secondary outcome measure was the correlation of the results of the primary measure with the dyes used to facilitate ILM peeling. Results: Glial and/or neuronal cells were detected on the retinal surface of the ILM in 10 of 31 (32{\%}) of the MH ILM specimens and in 13 of 20 (65{\%}) of the ILM peeled after ERM excision; the difference was significant (P = 0.02). There was no association between the presence of neuronal and glial cells with the type of dye used (P = 0.2). Of the 23 ILM specimens with cells attached to the retinal surface, 21 (91{\%}) were associated with clinical and/or histologic evidence of ERM and 2 (9{\%}) were not. The correlation between the presence of cells on the vitreous and the retinal surfaces of ILM was high (P",
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AU - Stappler, Theodore

AU - Romano, Mario R.

AU - Das, Ronald A.

AU - Hebbar, Gillian

AU - Prime, Wendy

AU - Heimann, Heinrich

AU - Gibran, Syed K.

AU - Sheridan, Carl M.

AU - Cheung, Yin Him

AU - Hiscott, Paul S.

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