Domain communication in Thermotoga maritima Arginine Binding Protein unraveled through protein dissection

Giovanni Smaldone, Nicole Balasco, Marilisa Vigorita, Alessia Ruggiero, Serena Cozzolino, Rita Berisio, Pompea Del Vecchio, Giuseppe Graziano, Luigi Vitagliano

Research output: Contribution to journalArticle

Abstract

Substrate binding proteins represent a large protein family that plays fundamental roles in selective transportation of metabolites across membrane. The function of these proteins relies on the relative motions of their two domains. Insights into domain communication in this class of proteins have been here collected using Thermotoga maritima Arginine Binding Protein (TmArgBP) as model system. TmArgBP was dissected into two domains (D1 and D2) that were exhaustively characterized using a repertoire of different experimental and computational techniques. Indeed, stability, crystalline structure, ability to recognize the arginine substrate, and dynamics of the two individual domains have been here studied. Present data demonstrate that, although in the parent protein both D1 and D2 cooperate for the arginine anchoring; only D1 is intrinsically able to bind the substrate. The implications of this finding on the mechanism of arginine binding and release by TmArgBP have been discussed. Interestingly, both D1 and D2 retain the remarkable thermal/chemical stability of the parent protein. The analysis of the structural and dynamic properties of TmArgBP and of the individual domains highlights possible routes of domain communication. Finally, this study generated two interesting molecular tools, the two stable isolated domains that could be used in future investigations.

Original languageEnglish
Pages (from-to)758-769
Number of pages12
JournalInternational Journal of Biological Macromolecules
Volume119
DOIs
Publication statusPublished - Nov 1 2018

Keywords

  • Biosensor
  • Diagnostic tool
  • Differential scanning calorimetry
  • Domain-domain communication
  • Protein dissection
  • Protein structure-stability

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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    Smaldone, G., Balasco, N., Vigorita, M., Ruggiero, A., Cozzolino, S., Berisio, R., Del Vecchio, P., Graziano, G., & Vitagliano, L. (2018). Domain communication in Thermotoga maritima Arginine Binding Protein unraveled through protein dissection. International Journal of Biological Macromolecules, 119, 758-769. https://doi.org/10.1016/j.ijbiomac.2018.07.172