TY - JOUR
T1 - Domain mobility as probed by small-angle X-ray scattering may account for substrate access to the active site of two copper-dependent amine oxidases
AU - Dainese, Enrico
AU - Sabatucci, Annalaura
AU - Pintus, Francesca
AU - Medda, Rosaria
AU - Angelucci, Clotilde Beatrice
AU - Floris, Giovanni
AU - Maccarrone, Mauro
PY - 2014
Y1 - 2014
N2 - Amine oxidases are a family of dimeric enzymes that contain one copper(II) ion and one 2,4,5-trihydroxyphenyalanine quinone per subunit. Here, the low-resolution structures of two Cu/TPQ amine oxidases from lentil (Lens esculenta) seedlings and from Euphorbia characias latex have been determined in solution by small-angle X-ray scattering. The active site of these enzymes is highly buried and requires a conformational change to allow substrate access. The study suggests that the funnel-shaped cavity located between the D3 and D4 domains is narrower within the crystal structure, whereas in solution the D3 domain could undergo movement resulting in a protein conformational change that is likely to lead to easier substrate access.
AB - Amine oxidases are a family of dimeric enzymes that contain one copper(II) ion and one 2,4,5-trihydroxyphenyalanine quinone per subunit. Here, the low-resolution structures of two Cu/TPQ amine oxidases from lentil (Lens esculenta) seedlings and from Euphorbia characias latex have been determined in solution by small-angle X-ray scattering. The active site of these enzymes is highly buried and requires a conformational change to allow substrate access. The study suggests that the funnel-shaped cavity located between the D3 and D4 domains is narrower within the crystal structure, whereas in solution the D3 domain could undergo movement resulting in a protein conformational change that is likely to lead to easier substrate access.
KW - amine oxidase
KW - conformational change
KW - copper
KW - domain movement
KW - SAXS
KW - TOPA
KW - TPQ
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U2 - 10.1107/S1399004714012140
DO - 10.1107/S1399004714012140
M3 - Article
C2 - 25084330
AN - SCOPUS:84905455006
VL - 70
SP - 2101
EP - 2110
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
SN - 0907-4449
IS - 8
ER -