Abstract
Background: Donor/recipient mixed chimerism has been reported to be associated with an increased risk of graft failure in patients with β-thalassemia given a bone marrow transplant. We investigated the relationship between the degree of mixed chimerism over time and clinical outcome of children undergoing cord blood transplantation for β-thalassemia. Design and Methods: Twenty-seven consecutive children given a cord blood transplant from a related donor were analyzed by short tandem repeat polymerase chain reaction and their chimerism results were compared with those of 79 consecutive patients who received a bone marrow transplant from either a relative (RD-BMT, n = 42) or an unrelated donor (UD-BMT, n = 37). Cord blood and bone marrow recipients received comparable preparative regimens. Results: All cord blood recipients engrafted and displayed mixed chimerism early after transplantation; 13/27 converted to full donor chimerism over time, while 14 maintained stable mixed chimerism; all patients are alive and transfusion-independent. Twenty-four of the 79 bone marrow-recipients (12 UD- and 12 RD-BMT) exhibited full donor chimerism at all time points examined, 4/79 (2 UD- and 2 RD-BMT) did not engraft and 51/79 (23 UD- and 28 RD-BMT) displayed mixed chimerism at the time of hematologic reconstitution. Forty of 51 bone marrow recipients with mixed chimerism converted to full donor chimerism (17 UD- and 23 RD-BMT), 3/51 maintained stable mixed chimerism (1 UD- and 2 RD-BMT), while 8/51 (5 UD- and 3 RD-BMT) progressively lost the graft, and became transfusion-dependent again. Conclusions: Mixed chimerism is a frequent event and does not predict the occurrence of graft failure in children with β-thalassemia given a cord blood transplant from a relative.
| Original language | English |
|---|---|
| Pages (from-to) | 1859-1867 |
| Number of pages | 9 |
| Journal | Haematologica |
| Volume | 93 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 2008 |
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Keywords
- β-thalassemia
- Cord blood transplantation
- Donor/recipient mixed chimerism
- Graft failure
- MC
- Tolerance
ASJC Scopus subject areas
- Hematology
Cite this
Donor/recipient mixed chimerism does not predict graft failure in children with β-thalassemia given an allogeneic cord blood transplant from an HLA-identical sibling. / Lisini, Daniela; Zecca, Marco; Giorgiani, Giovanna; Montagna, Daniela; Cristantielli, Rosaria; Labirio, Massimo; Grignani, Pierangela; Previderè, Carlo; Di Cesare-Merlone, Alessandra; Amendola, Giovanni; Bergami, Elena; Mastronuzzi, Angela; Maccario, Rita; Locatelli, Franco.
In: Haematologica, Vol. 93, No. 12, 12.2008, p. 1859-1867.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Donor/recipient mixed chimerism does not predict graft failure in children with β-thalassemia given an allogeneic cord blood transplant from an HLA-identical sibling
AU - Lisini, Daniela
AU - Zecca, Marco
AU - Giorgiani, Giovanna
AU - Montagna, Daniela
AU - Cristantielli, Rosaria
AU - Labirio, Massimo
AU - Grignani, Pierangela
AU - Previderè, Carlo
AU - Di Cesare-Merlone, Alessandra
AU - Amendola, Giovanni
AU - Bergami, Elena
AU - Mastronuzzi, Angela
AU - Maccario, Rita
AU - Locatelli, Franco
PY - 2008/12
Y1 - 2008/12
N2 - Background: Donor/recipient mixed chimerism has been reported to be associated with an increased risk of graft failure in patients with β-thalassemia given a bone marrow transplant. We investigated the relationship between the degree of mixed chimerism over time and clinical outcome of children undergoing cord blood transplantation for β-thalassemia. Design and Methods: Twenty-seven consecutive children given a cord blood transplant from a related donor were analyzed by short tandem repeat polymerase chain reaction and their chimerism results were compared with those of 79 consecutive patients who received a bone marrow transplant from either a relative (RD-BMT, n = 42) or an unrelated donor (UD-BMT, n = 37). Cord blood and bone marrow recipients received comparable preparative regimens. Results: All cord blood recipients engrafted and displayed mixed chimerism early after transplantation; 13/27 converted to full donor chimerism over time, while 14 maintained stable mixed chimerism; all patients are alive and transfusion-independent. Twenty-four of the 79 bone marrow-recipients (12 UD- and 12 RD-BMT) exhibited full donor chimerism at all time points examined, 4/79 (2 UD- and 2 RD-BMT) did not engraft and 51/79 (23 UD- and 28 RD-BMT) displayed mixed chimerism at the time of hematologic reconstitution. Forty of 51 bone marrow recipients with mixed chimerism converted to full donor chimerism (17 UD- and 23 RD-BMT), 3/51 maintained stable mixed chimerism (1 UD- and 2 RD-BMT), while 8/51 (5 UD- and 3 RD-BMT) progressively lost the graft, and became transfusion-dependent again. Conclusions: Mixed chimerism is a frequent event and does not predict the occurrence of graft failure in children with β-thalassemia given a cord blood transplant from a relative.
AB - Background: Donor/recipient mixed chimerism has been reported to be associated with an increased risk of graft failure in patients with β-thalassemia given a bone marrow transplant. We investigated the relationship between the degree of mixed chimerism over time and clinical outcome of children undergoing cord blood transplantation for β-thalassemia. Design and Methods: Twenty-seven consecutive children given a cord blood transplant from a related donor were analyzed by short tandem repeat polymerase chain reaction and their chimerism results were compared with those of 79 consecutive patients who received a bone marrow transplant from either a relative (RD-BMT, n = 42) or an unrelated donor (UD-BMT, n = 37). Cord blood and bone marrow recipients received comparable preparative regimens. Results: All cord blood recipients engrafted and displayed mixed chimerism early after transplantation; 13/27 converted to full donor chimerism over time, while 14 maintained stable mixed chimerism; all patients are alive and transfusion-independent. Twenty-four of the 79 bone marrow-recipients (12 UD- and 12 RD-BMT) exhibited full donor chimerism at all time points examined, 4/79 (2 UD- and 2 RD-BMT) did not engraft and 51/79 (23 UD- and 28 RD-BMT) displayed mixed chimerism at the time of hematologic reconstitution. Forty of 51 bone marrow recipients with mixed chimerism converted to full donor chimerism (17 UD- and 23 RD-BMT), 3/51 maintained stable mixed chimerism (1 UD- and 2 RD-BMT), while 8/51 (5 UD- and 3 RD-BMT) progressively lost the graft, and became transfusion-dependent again. Conclusions: Mixed chimerism is a frequent event and does not predict the occurrence of graft failure in children with β-thalassemia given a cord blood transplant from a relative.
KW - β-thalassemia
KW - Cord blood transplantation
KW - Donor/recipient mixed chimerism
KW - Graft failure
KW - MC
KW - Tolerance
UR - http://www.scopus.com/inward/record.url?scp=57349083313&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57349083313&partnerID=8YFLogxK
U2 - 10.3324/haematol.13248
DO - 10.3324/haematol.13248
M3 - Article
C2 - 18945748
AN - SCOPUS:57349083313
VL - 93
SP - 1859
EP - 1867
JO - Haematologica
JF - Haematologica
SN - 0390-6078
IS - 12
ER -