Abstract
CD8+ T regulatory/suppressor cells (Treg)affect peripheral tolerance and may be involved in autoimmune diseases as well as in cancer. In view of our previous data showing the ability of DA to affect adaptive immune responses, we investigated the dopaminergic phenotype of human CD8+ Treg as well as the ability of DA to affect their generation and activity. Results show that CD8+ T cells express both D1-like and D2-like dopaminergic receptors (DR), tyrosine hydroxylase (TH), the rate-limiting enzyme in the synthesis of DA, and vesicular monoamine transporter (VMAT)2 and contain high levels of intracellular DA. Preferential upregulation of DR mRNA levels in the CD8+CD28- T cell compartment occurs during generation of CD8+ Treg, which is reduced by DA and by the D1-like DR agonist SKF-38393. DA and SKF-38393 also reduce the suppressive activity of CD8+ Treg on human peripheral blood mononuclear cells. Treg are crucial for tumor escape from the host immune system, thus the ability of DA to inhibits Treg function supports dopaminergic pathways as a druggable targets to develop original and innovative antitumor strategies.
| Original language | English |
|---|---|
| Pages (from-to) | 233-241 |
| Number of pages | 9 |
| Journal | Journal of Neuroimmunology |
| Volume | 332 |
| DOIs | |
| Publication status | Published - Jul 15 2019 |
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Keywords
- CD8+ Treg
- Dopamine
- Dopaminergic receptor
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Neurology
- Clinical Neurology
Cite this
Dopamine inhibits human CD8+ Treg function through D1-like dopaminergic receptors. / Nasi, Giorgia; Ahmed, Tanzeel; Rasini, Emanuela; Fenoglio, Daniela; Marino, Franca; Filaci, Gilberto; Cosentino, Marco.
In: Journal of Neuroimmunology, Vol. 332, 15.07.2019, p. 233-241.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dopamine inhibits human CD8+ Treg function through D1-like dopaminergic receptors
AU - Nasi, Giorgia
AU - Ahmed, Tanzeel
AU - Rasini, Emanuela
AU - Fenoglio, Daniela
AU - Marino, Franca
AU - Filaci, Gilberto
AU - Cosentino, Marco
PY - 2019/7/15
Y1 - 2019/7/15
N2 - CD8+ T regulatory/suppressor cells (Treg)affect peripheral tolerance and may be involved in autoimmune diseases as well as in cancer. In view of our previous data showing the ability of DA to affect adaptive immune responses, we investigated the dopaminergic phenotype of human CD8+ Treg as well as the ability of DA to affect their generation and activity. Results show that CD8+ T cells express both D1-like and D2-like dopaminergic receptors (DR), tyrosine hydroxylase (TH), the rate-limiting enzyme in the synthesis of DA, and vesicular monoamine transporter (VMAT)2 and contain high levels of intracellular DA. Preferential upregulation of DR mRNA levels in the CD8+CD28- T cell compartment occurs during generation of CD8+ Treg, which is reduced by DA and by the D1-like DR agonist SKF-38393. DA and SKF-38393 also reduce the suppressive activity of CD8+ Treg on human peripheral blood mononuclear cells. Treg are crucial for tumor escape from the host immune system, thus the ability of DA to inhibits Treg function supports dopaminergic pathways as a druggable targets to develop original and innovative antitumor strategies.
AB - CD8+ T regulatory/suppressor cells (Treg)affect peripheral tolerance and may be involved in autoimmune diseases as well as in cancer. In view of our previous data showing the ability of DA to affect adaptive immune responses, we investigated the dopaminergic phenotype of human CD8+ Treg as well as the ability of DA to affect their generation and activity. Results show that CD8+ T cells express both D1-like and D2-like dopaminergic receptors (DR), tyrosine hydroxylase (TH), the rate-limiting enzyme in the synthesis of DA, and vesicular monoamine transporter (VMAT)2 and contain high levels of intracellular DA. Preferential upregulation of DR mRNA levels in the CD8+CD28- T cell compartment occurs during generation of CD8+ Treg, which is reduced by DA and by the D1-like DR agonist SKF-38393. DA and SKF-38393 also reduce the suppressive activity of CD8+ Treg on human peripheral blood mononuclear cells. Treg are crucial for tumor escape from the host immune system, thus the ability of DA to inhibits Treg function supports dopaminergic pathways as a druggable targets to develop original and innovative antitumor strategies.
KW - CD8+ Treg
KW - Dopamine
KW - Dopaminergic receptor
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UR - http://www.scopus.com/inward/citedby.url?scp=85063758013&partnerID=8YFLogxK
U2 - 10.1016/j.jneuroim.2019.02.007
DO - 10.1016/j.jneuroim.2019.02.007
M3 - Article
C2 - 30954278
AN - SCOPUS:85063758013
VL - 332
SP - 233
EP - 241
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
ER -