Abstract
Zinc ions exert an inhibitory effect on Ca2+/Mg2+-dependent endonuclease which is supposed to be responsible for the fragmentation of DNA during apoptosis. In the experimental system we used, that is HeLa cells treated with VP-16, the protection from internucleosomal DNA degradation is modulated by Zn concentration and appears to be dependent on the time after treatment. This effect does not prevent cell death or occurrence of apoptotic parameters, suggesting that DNA ladder appearance is not a crucial event in apoptosis. The activation of poly(ADP-ribose)polymerase following the administration of VP-16, is not observed in cells in which DNA fragmentation has been abolished by zinc , supporting the hypothesis that this event is regulated by the appearance of small-sized DNA fragments.
| Original language | English |
|---|---|
| Pages (from-to) | 101-107 |
| Number of pages | 7 |
| Journal | Cell Death and Differentiation |
| Volume | 1 |
| Issue number | 2 |
| Publication status | Published - 1994 |
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Keywords
- Activity gel
- Apoplosis
- Cytofluorimetric analysis
- DNA fragmentation
- Etoposide
- Poly(ADP-ribose)polymerase
- Zinc
ASJC Scopus subject areas
- Cell Biology
Cite this
Dose-dependent zinc inhibition of DNA ladder in apoptotic HeLa cells regulates the activity of poly(ADP-ribose) polymerase and does not protect from death induced by VP-16. / Guano, Fulvio; Bernardi, Rosa; Negri, Claudia; Donzelli, Maddalena; Prosperi, Ennio; Ricotti, Giulia Astaldi; Scovassi, A. Ivana.
In: Cell Death and Differentiation, Vol. 1, No. 2, 1994, p. 101-107.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dose-dependent zinc inhibition of DNA ladder in apoptotic HeLa cells regulates the activity of poly(ADP-ribose) polymerase and does not protect from death induced by VP-16
AU - Guano, Fulvio
AU - Bernardi, Rosa
AU - Negri, Claudia
AU - Donzelli, Maddalena
AU - Prosperi, Ennio
AU - Ricotti, Giulia Astaldi
AU - Scovassi, A. Ivana
PY - 1994
Y1 - 1994
N2 - Zinc ions exert an inhibitory effect on Ca2+/Mg2+-dependent endonuclease which is supposed to be responsible for the fragmentation of DNA during apoptosis. In the experimental system we used, that is HeLa cells treated with VP-16, the protection from internucleosomal DNA degradation is modulated by Zn concentration and appears to be dependent on the time after treatment. This effect does not prevent cell death or occurrence of apoptotic parameters, suggesting that DNA ladder appearance is not a crucial event in apoptosis. The activation of poly(ADP-ribose)polymerase following the administration of VP-16, is not observed in cells in which DNA fragmentation has been abolished by zinc , supporting the hypothesis that this event is regulated by the appearance of small-sized DNA fragments.
AB - Zinc ions exert an inhibitory effect on Ca2+/Mg2+-dependent endonuclease which is supposed to be responsible for the fragmentation of DNA during apoptosis. In the experimental system we used, that is HeLa cells treated with VP-16, the protection from internucleosomal DNA degradation is modulated by Zn concentration and appears to be dependent on the time after treatment. This effect does not prevent cell death or occurrence of apoptotic parameters, suggesting that DNA ladder appearance is not a crucial event in apoptosis. The activation of poly(ADP-ribose)polymerase following the administration of VP-16, is not observed in cells in which DNA fragmentation has been abolished by zinc , supporting the hypothesis that this event is regulated by the appearance of small-sized DNA fragments.
KW - Activity gel
KW - Apoplosis
KW - Cytofluorimetric analysis
KW - DNA fragmentation
KW - Etoposide
KW - Poly(ADP-ribose)polymerase
KW - Zinc
UR - http://www.scopus.com/inward/record.url?scp=0028121488&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028121488&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0028121488
VL - 1
SP - 101
EP - 107
JO - Cell Death and Differentiation
JF - Cell Death and Differentiation
SN - 1350-9047
IS - 2
ER -