Dose-dependent zinc inhibition of DNA ladder in apoptotic HeLa cells regulates the activity of poly(ADP-ribose) polymerase and does not protect from death induced by VP-16

Fulvio Guano; Rosa Bernardi; Claudia Negri; Maddalena Donzelli; Ennio Prosperi; Giulia Astaldi Ricotti; A. Ivana Scovassi

Dose-dependent zinc inhibition of DNA ladder in apoptotic HeLa cells regulates the activity of poly(ADP-ribose) polymerase and does not protect from death induced by VP-16

Fulvio Guano, Rosa Bernardi, Claudia Negri, Maddalena Donzelli, Ennio Prosperi, Giulia Astaldi Ricotti, A. Ivana Scovassi

Research output: Contribution to journal › Article › peer-review

Abstract

Zinc ions exert an inhibitory effect on Ca²⁺/Mg²⁺-dependent endonuclease which is supposed to be responsible for the fragmentation of DNA during apoptosis. In the experimental system we used, that is HeLa cells treated with VP-16, the protection from internucleosomal DNA degradation is modulated by Zn concentration and appears to be dependent on the time after treatment. This effect does not prevent cell death or occurrence of apoptotic parameters, suggesting that DNA ladder appearance is not a crucial event in apoptosis. The activation of poly(ADP-ribose)polymerase following the administration of VP-16, is not observed in cells in which DNA fragmentation has been abolished by zinc , supporting the hypothesis that this event is regulated by the appearance of small-sized DNA fragments.

Original language	English
Pages (from-to)	101-107
Number of pages	7
Journal	Cell Death and Differentiation
Volume	1
Issue number	2
Publication status	Published - 1994

Keywords

Activity gel
Apoplosis
Cytofluorimetric analysis
DNA fragmentation
Etoposide
Poly(ADP-ribose)polymerase
Zinc

ASJC Scopus subject areas

Cell Biology

Cite this

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title = "Dose-dependent zinc inhibition of DNA ladder in apoptotic HeLa cells regulates the activity of poly(ADP-ribose) polymerase and does not protect from death induced by VP-16",

abstract = "Zinc ions exert an inhibitory effect on Ca2+/Mg2+-dependent endonuclease which is supposed to be responsible for the fragmentation of DNA during apoptosis. In the experimental system we used, that is HeLa cells treated with VP-16, the protection from internucleosomal DNA degradation is modulated by Zn concentration and appears to be dependent on the time after treatment. This effect does not prevent cell death or occurrence of apoptotic parameters, suggesting that DNA ladder appearance is not a crucial event in apoptosis. The activation of poly(ADP-ribose)polymerase following the administration of VP-16, is not observed in cells in which DNA fragmentation has been abolished by zinc , supporting the hypothesis that this event is regulated by the appearance of small-sized DNA fragments.",

keywords = "Activity gel, Apoplosis, Cytofluorimetric analysis, DNA fragmentation, Etoposide, Poly(ADP-ribose)polymerase, Zinc",

author = "Fulvio Guano and Rosa Bernardi and Claudia Negri and Maddalena Donzelli and Ennio Prosperi and Ricotti, {Giulia Astaldi} and Scovassi, {A. Ivana}",

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journal = "Cell Death and Differentiation",

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T1 - Dose-dependent zinc inhibition of DNA ladder in apoptotic HeLa cells regulates the activity of poly(ADP-ribose) polymerase and does not protect from death induced by VP-16

AU - Guano, Fulvio

AU - Bernardi, Rosa

AU - Negri, Claudia

AU - Donzelli, Maddalena

AU - Prosperi, Ennio

AU - Ricotti, Giulia Astaldi

AU - Scovassi, A. Ivana

PY - 1994

Y1 - 1994

N2 - Zinc ions exert an inhibitory effect on Ca2+/Mg2+-dependent endonuclease which is supposed to be responsible for the fragmentation of DNA during apoptosis. In the experimental system we used, that is HeLa cells treated with VP-16, the protection from internucleosomal DNA degradation is modulated by Zn concentration and appears to be dependent on the time after treatment. This effect does not prevent cell death or occurrence of apoptotic parameters, suggesting that DNA ladder appearance is not a crucial event in apoptosis. The activation of poly(ADP-ribose)polymerase following the administration of VP-16, is not observed in cells in which DNA fragmentation has been abolished by zinc , supporting the hypothesis that this event is regulated by the appearance of small-sized DNA fragments.

AB - Zinc ions exert an inhibitory effect on Ca2+/Mg2+-dependent endonuclease which is supposed to be responsible for the fragmentation of DNA during apoptosis. In the experimental system we used, that is HeLa cells treated with VP-16, the protection from internucleosomal DNA degradation is modulated by Zn concentration and appears to be dependent on the time after treatment. This effect does not prevent cell death or occurrence of apoptotic parameters, suggesting that DNA ladder appearance is not a crucial event in apoptosis. The activation of poly(ADP-ribose)polymerase following the administration of VP-16, is not observed in cells in which DNA fragmentation has been abolished by zinc , supporting the hypothesis that this event is regulated by the appearance of small-sized DNA fragments.

KW - Activity gel

KW - Apoplosis

KW - Cytofluorimetric analysis

KW - DNA fragmentation

KW - Etoposide

KW - Poly(ADP-ribose)polymerase

KW - Zinc

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Dose-dependent zinc inhibition of DNA ladder in apoptotic HeLa cells regulates the activity of poly(ADP-ribose) polymerase and does not protect from death induced by VP-16

Abstract

Keywords

ASJC Scopus subject areas

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