Double-stranded internucleosomal cleavage of apoptotic DNA is dependent on the degree of differentiation in muscle cells

Gian Maria Fimia, Vanesa Gottifredi, Claudio Passananti, Rossella Maionel

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Apoptotic cell death has been correlated to DNA fragmentation into discrete segments corresponding to the length of nucleosomal protected fragments of 180-200 base pairs or multiples of it. This DNA degradation has been ascribed to endonuclease activity that cleaves internucleosomally, thus giving rise to a ladder distribution upon electrophoretic migration. This strict correlation was, however, shown to have notable exceptions, since in some cases only single strand cleavage in the internucleosomal DNA regions has been observed (Tomei, D. L., Shapiro, P. J., and Cope, O. F. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 853-857). In the present work we show that mouse muscle cells, able to differentiate in vitro, if subjected to apoptosis present no DNA degradation into ladder form unless differentiation is previously induced. Furthermore, C3H/10T1/2 fibroblast cells, known to undergo apoptosis without DNA ladder formation, if converted to a myogenic program by MyoD expression, display internucleosomal DNA degradation upon induction of differentiation.

Original languageEnglish
Pages (from-to)15575-15579
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number26
DOIs
Publication statusPublished - 1996

Fingerprint

DNA Cleavage
Muscle Cells
Muscle
Cells
DNA
Ladders
Degradation
Apoptosis
Endonucleases
DNA Fragmentation
Base Pairing
Cell death
Fibroblasts
Cell Death

ASJC Scopus subject areas

  • Biochemistry

Cite this

Double-stranded internucleosomal cleavage of apoptotic DNA is dependent on the degree of differentiation in muscle cells. / Fimia, Gian Maria; Gottifredi, Vanesa; Passananti, Claudio; Maionel, Rossella.

In: Journal of Biological Chemistry, Vol. 271, No. 26, 1996, p. 15575-15579.

Research output: Contribution to journalArticle

Fimia, Gian Maria ; Gottifredi, Vanesa ; Passananti, Claudio ; Maionel, Rossella. / Double-stranded internucleosomal cleavage of apoptotic DNA is dependent on the degree of differentiation in muscle cells. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 26. pp. 15575-15579.
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