Double stranded promoter region of BRAF undergoes to structural rearrangement in nearly physiological conditions

Maria Laura Greco, Marco Folini, Claudia Sissi

Research output: Contribution to journalArticle

Abstract

The folding of oncogene promoters into non-canonical DNA secondary structures is considered a strategy to control gene expression. Herein, we focused on a 30 bases sequence located upstream of the transcription start site of BRAF (Braf-176) that contains 80% of guanines. We analyzed the structural behavior of the G- and C-rich strands. By the use of spectroscopic and electrophoretic techniques we confirmed that they actually fold into a predominant antiparallel G-quadruplex and into an i-motif, respectively, and that they can coexist at nearly physiological conditions. Finally, the influence of several factors (KCl, pH, PEG200) on the conversion of the double stranded form of the oncogene promoter into the two above mentioned non-canonical structures has been explored.

Original languageEnglish
Pages (from-to)2117-2123
Number of pages7
JournalFEBS Letters
Volume589
Issue number16
DOIs
Publication statusPublished - Feb 17 2015

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Keywords

  • BRAF
  • G-quadruplex
  • i-motif
  • Oncogene

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology
  • Medicine(all)

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