Down-regulated miRs specifically correlate with non-cardial gastric cancers and Lauren's classification system

L. Lorenzon, C. Cippitelli, R. Avantifiori, S. Uccini, D. French, M. R. Torrisi, D. Ranieri, P. Mercantini, V. Canu, G. Blandino, M. Cavallini

Research output: Contribution to journalArticlepeer-review


BACKGROUND AND OBJECTIVES: Gastric cancers are usually characterized using Lauren's classification into intestinal and diffuse types. We previously documented the down-modulation of miR31, miR148a, miR204, and miR375 in gastric cancers. We aimed this manuscript to investigate these miRs with the end-points of diagnosis, Lauren's classification and prognosis. METHODS: A total of 117 resected non-cardial adenocarcinomas were evaluated for miRs' expressions. The performance of miRs' expressions for cancer diagnosis was tested using ROC curves. Logistic regression was conducted with the end-point of Lauren's classification. Kaplan-Meier and Cox analyses were performed for OS, DFS, and DSS. miRs' targets were reviewed using PRISMA method and BCL-2 was further investigated in cell lines. RESULTS: ROC curves documented that miRs' down-modulation was significant in differentiating cancer versus normal tissues. Diffuse type cancers were associated with female sex, young age, and miR375 higher expression. We confirmed BCL-2 as a miR204 target. However, survival analyses confirmed the pathologic criteria (advanced stages, LNR, and low LNH) as the significant variables correlated to worse prognosis. CONCLUSIONS: The down-modulation of miR31, miR148a, miR204, and miR375 is significantly associated with non-cardial gastric cancers and miR375 is specifically linked to Lauren's classification. Nevertheless, standard pathological features display as the independent variables associated with worse prognosis.
Original languageEnglish
Pages (from-to)184-194
Number of pages11
JournalJournal of Surgical Oncology
Issue number2
Publication statusPublished - Aug 1 2017


  • Adenocarcinoma/classification/genetics/metabolism
  • Adult
  • Age Factors
  • Aged
  • Aged, 80 and over
  • Cell Line, Tumor
  • Down-Regulation
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Janus Kinase 2/genetics/metabolism
  • Male
  • MicroRNAs/genetics/metabolism
  • Middle Aged
  • Prognosis
  • Protein-Serine-Threonine Kinases/genetics/metabolism
  • Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
  • ROC Curve
  • Repressor Proteins/genetics/metabolism
  • Retrospective Studies
  • Sensitivity and Specificity
  • Sex Factors
  • Stomach Neoplasms/classification/genetics/metabolism
  • Lauren classification
  • gastric cancer
  • miR148a
  • miR204
  • miR31
  • miR375
  • non-cardial gastric cancer


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