Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis

Paulo Ornellas, Antonio Augusto Ornellas, Clizia Chinello, Erica Gianazza, Veronica Mainini, Marta Cazzaniga, Denise Abreu Pereira, Vanessa Sandim, Ana Sheila Cypriano, Leandro Koifman, Paulo Cesar Barbosa Da Silva, Gilda Alves, Fulvio Magni

Research output: Contribution to journalArticle

Abstract

Purpose:To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and Methods:Plasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identified from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS. Results:A cluster of 2 peptides (A=m/z 1897.22 ± 9 Da and B=m/z 2021.99 ± 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specificity, respectively. The cluster also showed very high sensitivity (100%) and specificity (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specificity of 80% and 97%, respectively. These two peptides were identified by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins. Conclusions:The results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools.

Original languageEnglish
Pages (from-to)739-749
Number of pages11
JournalInternational Braz J Urol
Volume38
Issue number6
DOIs
Publication statusPublished - Nov 2012

Keywords

  • Carcinoma
  • Complement c3b inactivator proteins
  • Penis
  • Penis cancer
  • Plasma
  • Proteomics
  • Squamous cell

ASJC Scopus subject areas

  • Urology

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  • Cite this

    Ornellas, P., Ornellas, A. A., Chinello, C., Gianazza, E., Mainini, V., Cazzaniga, M., Pereira, D. A., Sandim, V., Cypriano, A. S., Koifman, L., Da Silva, P. C. B., Alves, G., & Magni, F. (2012). Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis. International Braz J Urol, 38(6), 739-749. https://doi.org/10.1590/1677-553820133806739