Downregulation of CD40 ligand response in monocytes from sepsis patients

Anna Sinistro, Cristiana Almerighi, Chiara Ciaprini, Silvia Natoli, Emanuele Sussarello, Sara Di Fino, Francesca Calò-Carducci, Giovanni Rocchi, Alberto Bergamini

Research output: Contribution to journalArticlepeer-review

Abstract

It has been suggested that a defective adaptive immune response contributes to septic immunosuppression. Here, the response of monocytes to CD40 ligand (CD40L) for patients with sepsis due to infection with gram-negative organisms has been analyzed. Compared to cells from controls, monocytes from septic patients showed significantly reduced production of tumor necrosis factor alpha, interleukin-1β (IL-1β), and IL-12 and were unable to acquire high levels of CD80 and CD86 molecules. These alterations were observed at the onset of sepsis and persisted at day 7. However, the ability of monocytes to respond to CD40L stimulation was partially but significantly restored in cells from patients who recovered from sepsis. In addition, costimulation of autologous CD4+ T lymphocytes by CD40L-activated monocytes from septic patients failed to induce cell proliferation and gamma interferon production. Finally, the ability of CD40L to rescue monocytes from apoptosis was severely impaired. We conclude that downregulation of the CD40L response may be an appropriate model for the monocyte alteration observed during septic immunosuppression and may help in the development of novel therapeutic strategies.

Original languageEnglish
Pages (from-to)1851-1858
Number of pages8
JournalClinical and Vaccine Immunology
Volume15
Issue number12
DOIs
Publication statusPublished - Dec 2008

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

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