Downregulation of HLA class I antigen expression in CD4+ T lymphocytes from HIV type 1-infected individuals

Francesco Puppo, Sabrina Brenci, Ornella Bosco, Lorella Lanza, Sergio Barocci, Arcangelo Nocera, Massimo Ghio, Paola Contini, Maurizio Setti, Marco Scudeletti, Francesco Indiveri

Research output: Contribution to journalArticlepeer-review

Abstract

The expression of HLA class I antigens is downregulated in CD4+ T cells following in vitro HIV-1 infection. We determined whether the expression of HLA class I antigens is downmodulated in peripheral blood lymphocytes (PBLs) of HIV-l-positive subjects and whether this defect correlates with disease progression. A cohort of 62 HIV-1-seropositive individuals in different stages of disease was studied. Among these, four subjects were evaluated at yearly intervals for 6 years. The expression of HLA class I, HLA class II, and CD38 antigens was analyzed in PBLs and in CD4+ and CD8+ T lymphocyte subpopulations. The percentage of HLA class I-positive cells and the membrane density of HLA class I antigens were significantly lower in PBLs from HIV-l- positive individuals than in PBLs from HIV-negative controls, proportionally decreased with disease progression, and significantly correlated with the decrease in CD4+ T lymphocytes. Furthermore, the percentage of HLA class I- positive cells and the membrane density of HLA class I antigens were significantly lower in CD4+ T lymphocytes from AIDS patients with respect to CD4+ T lymphocytes from HIV-negative controls and to CD8+ T lymphocytes from HIV-negative controls and AIDS patients. By contrast, the expression of HLA class II and CD38 antigens was upregulated in CD4+ and CD8+ T lymphocytes from HIV-1-positive subjects. The defective expression of HLA class I antigens could impair the lysis of HIV-infected CD4+ cells by virus- specific HLA class I-restricted cytotoxic T lymphocytes and contribute to the progression of disease.

Original languageEnglish
Pages (from-to)1509-1516
Number of pages8
JournalAIDS Research and Human Retroviruses
Volume13
Issue number17
Publication statusPublished - Nov 20 1997

ASJC Scopus subject areas

  • Immunology
  • Virology

Fingerprint Dive into the research topics of 'Downregulation of HLA class I antigen expression in CD4+ T lymphocytes from HIV type 1-infected individuals'. Together they form a unique fingerprint.

Cite this