Downregulation of HLA class I antigen expression in CD4+ T lymphocytes from HIV type 1-infected individuals

Francesco Puppo, Sabrina Brenci, Ornella Bosco, Lorella Lanza, Sergio Barocci, Arcangelo Nocera, Massimo Ghio, Paola Contini, Maurizio Setti, Marco Scudeletti, Francesco Indiveri

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Abstract

The expression of HLA class I antigens is downregulated in CD4+ T cells following in vitro HIV-1 infection. We determined whether the expression of HLA class I antigens is downmodulated in peripheral blood lymphocytes (PBLs) of HIV-l-positive subjects and whether this defect correlates with disease progression. A cohort of 62 HIV-1-seropositive individuals in different stages of disease was studied. Among these, four subjects were evaluated at yearly intervals for 6 years. The expression of HLA class I, HLA class II, and CD38 antigens was analyzed in PBLs and in CD4+ and CD8+ T lymphocyte subpopulations. The percentage of HLA class I-positive cells and the membrane density of HLA class I antigens were significantly lower in PBLs from HIV-l- positive individuals than in PBLs from HIV-negative controls, proportionally decreased with disease progression, and significantly correlated with the decrease in CD4+ T lymphocytes. Furthermore, the percentage of HLA class I- positive cells and the membrane density of HLA class I antigens were significantly lower in CD4+ T lymphocytes from AIDS patients with respect to CD4+ T lymphocytes from HIV-negative controls and to CD8+ T lymphocytes from HIV-negative controls and AIDS patients. By contrast, the expression of HLA class II and CD38 antigens was upregulated in CD4+ and CD8+ T lymphocytes from HIV-1-positive subjects. The defective expression of HLA class I antigens could impair the lysis of HIV-infected CD4+ cells by virus- specific HLA class I-restricted cytotoxic T lymphocytes and contribute to the progression of disease.

Original languageEnglish
Pages (from-to)1509-1516
Number of pages8
JournalAIDS Research and Human Retroviruses
Volume13
Issue number17
Publication statusPublished - Nov 20 1997

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Histocompatibility Antigens Class I
HLA Antigens
HIV-1
Down-Regulation
T-Lymphocytes
HIV
CD38 Antigens
Lymphocytes
Disease Progression
Histocompatibility Antigens Class II
Acquired Immunodeficiency Syndrome
Cell Count
Cell Membrane
Lymphocyte Subsets
Cytotoxic T-Lymphocytes
HIV Infections
Viruses

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Puppo, F., Brenci, S., Bosco, O., Lanza, L., Barocci, S., Nocera, A., ... Indiveri, F. (1997). Downregulation of HLA class I antigen expression in CD4+ T lymphocytes from HIV type 1-infected individuals. AIDS Research and Human Retroviruses, 13(17), 1509-1516.

Downregulation of HLA class I antigen expression in CD4+ T lymphocytes from HIV type 1-infected individuals. / Puppo, Francesco; Brenci, Sabrina; Bosco, Ornella; Lanza, Lorella; Barocci, Sergio; Nocera, Arcangelo; Ghio, Massimo; Contini, Paola; Setti, Maurizio; Scudeletti, Marco; Indiveri, Francesco.

In: AIDS Research and Human Retroviruses, Vol. 13, No. 17, 20.11.1997, p. 1509-1516.

Research output: Contribution to journalArticle

Puppo, F, Brenci, S, Bosco, O, Lanza, L, Barocci, S, Nocera, A, Ghio, M, Contini, P, Setti, M, Scudeletti, M & Indiveri, F 1997, 'Downregulation of HLA class I antigen expression in CD4+ T lymphocytes from HIV type 1-infected individuals', AIDS Research and Human Retroviruses, vol. 13, no. 17, pp. 1509-1516.
Puppo F, Brenci S, Bosco O, Lanza L, Barocci S, Nocera A et al. Downregulation of HLA class I antigen expression in CD4+ T lymphocytes from HIV type 1-infected individuals. AIDS Research and Human Retroviruses. 1997 Nov 20;13(17):1509-1516.
Puppo, Francesco ; Brenci, Sabrina ; Bosco, Ornella ; Lanza, Lorella ; Barocci, Sergio ; Nocera, Arcangelo ; Ghio, Massimo ; Contini, Paola ; Setti, Maurizio ; Scudeletti, Marco ; Indiveri, Francesco. / Downregulation of HLA class I antigen expression in CD4+ T lymphocytes from HIV type 1-infected individuals. In: AIDS Research and Human Retroviruses. 1997 ; Vol. 13, No. 17. pp. 1509-1516.
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