Downregulation of human polymorphonuclear cell activities exerted by microorganisms belonging to the α2 subgroup of proteobacteria (afipia fs and rochallmaea henselae)

D. Fumarola, S. Pece, R. Fumarulo, R. Petruzzelli, B. Greco, G. Giuliani, A. B. Maffione, E. Jirillo

Research output: Contribution to journalArticle

Abstract

lntracellular pathogens have evolved effective mechanisms in order to survive in an intracellular environment, thus avoiding destruction by phagocytic cells. In this regard, a correlation between resistance to phagocytic killing and expression of pathogenic potency has been established. In this report, we have studied the interaction between human polymorphonuclear cells (PMN) and two gram-negative microorganisms, Afipia felis and Rochalimaea henselae, which belong to the α2 subgroup of the class Proteobacteria. A. felis has been previously proposed as the causative agent of Cat Scratch Disease (CSD), but several recent lines of evidence attribute a major role to R. henselae. Of note, CSD is a syndrome characterized by a chronic lymphoadenopathy, involving macrophages and endothelial cells with a progression towards a granulomatous process and/or angiogenesis. Since members of the α2 subgroup of Proteobacteria have the property to survive intracellularly, we have evaluated the effects exerted by A. felis and R.henselae on human PMN in terms of chemotaxis locomotion, degranulation and oxidative metabolism. Results will show an impairment of PMN activities as a consequence of the challenge with both microrganisms. In particular, inhibition of PMN oxidative function occurred either as result of a direct exposure to both A.felis and R. henselae or when PMN were primed by bacteria for the N-formyl-methionyl-leucyl-phenylalanine enhancement of the oxidative burst. These findings may account for the ability of A. felis and R. henselae to survive within PMN as expression of a further mechanism of pathogenic potency, influencing also the nature and the evolution of inflammatory response in the lesion sites.

Original languageEnglish
Pages (from-to)449-461
Number of pages13
JournalImmunopharmacology and Immunotoxicology
Volume16
Issue number3
DOIs
Publication statusPublished - 1994

Fingerprint

Afipia
Proteobacteria
Bartonella henselae
Microorganisms
Down-Regulation
N-Formylmethionine Leucyl-Phenylalanine
Macrophages
Endothelial cells
Pathogens
Metabolism
Cat-Scratch Disease
Bacteria
Felis
Aptitude
Respiratory Burst
Chemotaxis
Locomotion
Phagocytes
Endothelial Cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Downregulation of human polymorphonuclear cell activities exerted by microorganisms belonging to the α2 subgroup of proteobacteria (afipia fs and rochallmaea henselae). / Fumarola, D.; Pece, S.; Fumarulo, R.; Petruzzelli, R.; Greco, B.; Giuliani, G.; Maffione, A. B.; Jirillo, E.

In: Immunopharmacology and Immunotoxicology, Vol. 16, No. 3, 1994, p. 449-461.

Research output: Contribution to journalArticle

Fumarola, D. ; Pece, S. ; Fumarulo, R. ; Petruzzelli, R. ; Greco, B. ; Giuliani, G. ; Maffione, A. B. ; Jirillo, E. / Downregulation of human polymorphonuclear cell activities exerted by microorganisms belonging to the α2 subgroup of proteobacteria (afipia fs and rochallmaea henselae). In: Immunopharmacology and Immunotoxicology. 1994 ; Vol. 16, No. 3. pp. 449-461.
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AU - Fumarulo, R.

AU - Petruzzelli, R.

AU - Greco, B.

AU - Giuliani, G.

AU - Maffione, A. B.

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