Downregulation of map kinase activity signalled by HIV-1-gp120 coat protein in granular neurons and glial cells from rat cerebellum

Chiara Leoni, Andrea Menegon, Fabio Benfenati, Flavia Valtorta

Research output: Contribution to journalArticle

Abstract

We have studied the effect of gp120 coat protein from HIV-1 on tyrosine phosphorylation processes in primary cultures of granular neurons or glial cells from the cerebellum of neonatal rats. The extracellular application of recombinant gp120 (200 pM) was able to reduce the phosphotyrosine content and the immunoreactivity for active form-specific antibodies of MAP kinase. Whereas in neurons MAP kinase appeared to be the only protein whose phosphotyrosine content was decreased, in glial cultures the inhibitory effect of gp120 on tyrosine phosphorylation processes appeared to be more widespread. In neuronal cultures, the effect of the viral protein was prevented by the concomitant treatment with depolarizing agents.

Original languageEnglish
Pages (from-to)683-686
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume240
Issue number3
DOIs
Publication statusPublished - Nov 26 1997

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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