Abstract
We have studied the effect of gp120 coat protein from HIV-1 on tyrosine phosphorylation processes in primary cultures of granular neurons or glial cells from the cerebellum of neonatal rats. The extracellular application of recombinant gp120 (200 pM) was able to reduce the phosphotyrosine content and the immunoreactivity for active form-specific antibodies of MAP kinase. Whereas in neurons MAP kinase appeared to be the only protein whose phosphotyrosine content was decreased, in glial cultures the inhibitory effect of gp120 on tyrosine phosphorylation processes appeared to be more widespread. In neuronal cultures, the effect of the viral protein was prevented by the concomitant treatment with depolarizing agents.
| Original language | English |
|---|---|
| Pages (from-to) | 683-686 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 240 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Nov 26 1997 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Downregulation of map kinase activity signalled by HIV-1-gp120 coat protein in granular neurons and glial cells from rat cerebellum. / Leoni, Chiara; Menegon, Andrea; Benfenati, Fabio; Valtorta, Flavia.
In: Biochemical and Biophysical Research Communications, Vol. 240, No. 3, 26.11.1997, p. 683-686.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Downregulation of map kinase activity signalled by HIV-1-gp120 coat protein in granular neurons and glial cells from rat cerebellum
AU - Leoni, Chiara
AU - Menegon, Andrea
AU - Benfenati, Fabio
AU - Valtorta, Flavia
PY - 1997/11/26
Y1 - 1997/11/26
N2 - We have studied the effect of gp120 coat protein from HIV-1 on tyrosine phosphorylation processes in primary cultures of granular neurons or glial cells from the cerebellum of neonatal rats. The extracellular application of recombinant gp120 (200 pM) was able to reduce the phosphotyrosine content and the immunoreactivity for active form-specific antibodies of MAP kinase. Whereas in neurons MAP kinase appeared to be the only protein whose phosphotyrosine content was decreased, in glial cultures the inhibitory effect of gp120 on tyrosine phosphorylation processes appeared to be more widespread. In neuronal cultures, the effect of the viral protein was prevented by the concomitant treatment with depolarizing agents.
AB - We have studied the effect of gp120 coat protein from HIV-1 on tyrosine phosphorylation processes in primary cultures of granular neurons or glial cells from the cerebellum of neonatal rats. The extracellular application of recombinant gp120 (200 pM) was able to reduce the phosphotyrosine content and the immunoreactivity for active form-specific antibodies of MAP kinase. Whereas in neurons MAP kinase appeared to be the only protein whose phosphotyrosine content was decreased, in glial cultures the inhibitory effect of gp120 on tyrosine phosphorylation processes appeared to be more widespread. In neuronal cultures, the effect of the viral protein was prevented by the concomitant treatment with depolarizing agents.
UR - http://www.scopus.com/inward/record.url?scp=0031587379&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031587379&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1997.7710
DO - 10.1006/bbrc.1997.7710
M3 - Article
C2 - 9398626
AN - SCOPUS:0031587379
VL - 240
SP - 683
EP - 686
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -