Downregulation of miR-410 targeting the cyclin B1 gene plays a role in pituitary gonadotroph tumors

Paula Müssnich, Gerald Raverot, Marie Lise Jaffrain-Rea, Filippo Fraggetta, Anne Wierinckx, Jacqueline Trouillas, Alfredo Fusco, Daniela D'Angelo

Research output: Contribution to journalArticle

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that act as posttranscriptional regulators of gene expression, and are frequently altered in human neoplasias. Here, we have analyzed the miRNA expression profile of human gonadotroph adenomas versus normal pituitary tissue using a miRNACHIP microarray. We demonstrate that miRNA-410 is downregulated in gonadotroph adenomas when compared with normal pituitary gland. We validate CCNB1 as target of miRNA-410 since its overexpression reduces CCNB1 at protein and mRNA levels, decreasing cell proliferation. In conclusion, our study suggess that the downregulation of miRNA-410 plays a role in the behavior of gonadotroph tumors.

Original languageEnglish
Pages (from-to)2590-2597
Number of pages8
JournalCell Cycle
Volume14
Issue number16
DOIs
Publication statusPublished - Jan 1 2015

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Keywords

  • Cyclin B1
  • miRNA-410
  • Pituitary adenomas

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Müssnich, P., Raverot, G., Jaffrain-Rea, M. L., Fraggetta, F., Wierinckx, A., Trouillas, J., Fusco, A., & D'Angelo, D. (2015). Downregulation of miR-410 targeting the cyclin B1 gene plays a role in pituitary gonadotroph tumors. Cell Cycle, 14(16), 2590-2597. https://doi.org/10.1080/15384101.2015.1064207