TY - JOUR
T1 - Downregulation of miR-410 targeting the cyclin B1 gene plays a role in pituitary gonadotroph tumors
AU - Müssnich, Paula
AU - Raverot, Gerald
AU - Jaffrain-Rea, Marie Lise
AU - Fraggetta, Filippo
AU - Wierinckx, Anne
AU - Trouillas, Jacqueline
AU - Fusco, Alfredo
AU - D'Angelo, Daniela
PY - 2015/1/1
Y1 - 2015/1/1
N2 - MicroRNAs (miRNAs) are small noncoding RNAs that act as posttranscriptional regulators of gene expression, and are frequently altered in human neoplasias. Here, we have analyzed the miRNA expression profile of human gonadotroph adenomas versus normal pituitary tissue using a miRNACHIP microarray. We demonstrate that miRNA-410 is downregulated in gonadotroph adenomas when compared with normal pituitary gland. We validate CCNB1 as target of miRNA-410 since its overexpression reduces CCNB1 at protein and mRNA levels, decreasing cell proliferation. In conclusion, our study suggess that the downregulation of miRNA-410 plays a role in the behavior of gonadotroph tumors.
AB - MicroRNAs (miRNAs) are small noncoding RNAs that act as posttranscriptional regulators of gene expression, and are frequently altered in human neoplasias. Here, we have analyzed the miRNA expression profile of human gonadotroph adenomas versus normal pituitary tissue using a miRNACHIP microarray. We demonstrate that miRNA-410 is downregulated in gonadotroph adenomas when compared with normal pituitary gland. We validate CCNB1 as target of miRNA-410 since its overexpression reduces CCNB1 at protein and mRNA levels, decreasing cell proliferation. In conclusion, our study suggess that the downregulation of miRNA-410 plays a role in the behavior of gonadotroph tumors.
KW - Cyclin B1
KW - miRNA-410
KW - Pituitary adenomas
UR - http://www.scopus.com/inward/record.url?scp=84943741686&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84943741686&partnerID=8YFLogxK
U2 - 10.1080/15384101.2015.1064207
DO - 10.1080/15384101.2015.1064207
M3 - Article
AN - SCOPUS:84943741686
VL - 14
SP - 2590
EP - 2597
JO - Cell Cycle
JF - Cell Cycle
SN - 1538-4101
IS - 16
ER -