Doxorubicin and Congo Red effectiveness on prion infectivity in golden Syrian hamster

Manuel Corato, Paolo Ogliari, Fabrizio Ceciliani, Emanuela Cova, Vincenzo Bellotti, Cristina Cereda, Giampaolo Merlini, Mauro Ceroni

Research output: Contribution to journalArticle

Abstract

The effect of doxorubicin and Congo Red on prion protein (PrP) infectivity in experimental scrapie was studied to better understand the effect of these compounds in prion diseases and to establish whether a dose-response correlation exists for Congo Red. This was performed in order to test the effectiveness of compounds that may easily be used in human prion diseases. Brain homogenate containing membrane bound PrPSc monomers was used as inoculum and was previously incubated with doxorubicin 10-3 M and with increasing concentrations of Congo Red ranging from 10-7 to 10-2 M. This study shows for the first time that doxorubicin, and confirms that Congo Red, may interact with pathological PrP monomers modifying their infectious properties. Pre-incubation of infected brain homogenate with Congo Red resulted in prolonged incubation time and survival, independently of Congo Red concentration (p

Original languageEnglish
Pages (from-to)2507-2512
Number of pages6
JournalAnticancer Research
Volume29
Issue number7
Publication statusPublished - Jul 2009

Keywords

  • Amyloid plaques
  • Congo Red
  • Doxorubicin
  • Prion disease

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'Doxorubicin and Congo Red effectiveness on prion infectivity in golden Syrian hamster'. Together they form a unique fingerprint.

  • Cite this