DR antigen expression on ovarian carcinoma cells does not correlate with their capacity to elicit an autologous proliferative response

Maria Di Bello, Valeria Lucchini, Stefania Chiari, Roberto Colleoni, Nicoletta Colombo, Alberto Mantovani, Paola Allavena

Research output: Contribution to journalArticlepeer-review

Abstract

Expression of HLA-DR antigens by purified preparations of human ovarian carcinoma cells freshly isolated from surgical specimens was examined in parallel with the capacity of tumor cells to elicit a blastogenic response from autologous lymphocytes in mixed lymphocyte-tumor culture (MLTC) assay. Of 21 tumor preparations, 11 (52%) reacted with monoclonal antibodies 279 and/or 949 specific for a monomorphic determinant of HLA-DR antigens, with heterogeneous positivity, ranging between 30% and 95%. In this series of patients positive MLTC occurred in 8/21 individual experiments. The HLA-DR expression was proportionally similar in tumors giving positive MLTC (4/8=50%) and negative MLTC (7/13=53%). The lack of correlation between DR expression on tumor cells and stimulatory activity in autologous MLTC and the fact that DR-negative tumors could induce lymphocyte stimulation, support the hypothesis that blastogenesis occurs upon recognition of tumor-associated antigens, different from DR molecules, possibly tumor-specific antigens.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalCancer Immunology, Immunotherapy
Volume27
Issue number1
DOIs
Publication statusPublished - Aug 1988

ASJC Scopus subject areas

  • Oncology
  • Immunology
  • Cancer Research

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