Dried blood and serum spots as a useful tool for sample storage to evaluate cancer biomarkers

Research output: Contribution to journalArticle

Abstract

Blood sample quality is crucial to ensure accurate downstream analyses such as real-time PCR or ELISA. Correct storage of biological materials is the starting point to achieve reproducible and reliable results. All samples should be treated in the same way from blood collection to storage. Depending on the analyses to be performed, whole blood and serum samples should be stored at-20 °C or-80 °C until use. Blood/serum samples should also be aliquoted to avoid multiple freeze-thawing. Another important issue is the sample conditions during shipment from one laboratory to another. If dry ice is not available or the shipment takes longer than a few days, alternative approaches are needed. One option is to use filter paper for blood collection. Here, we propose a method for blood and serum sample collection that takes advantage of dried blood spots (DBS) and dried serum spots (DSS). We developed the procedure to extract DNA from DBS for the downstream evaluation of some single nucleotide polymorphisms (SNPs) by real time PCR. We also optimized an ELISA assay starting from proteins eluted from DSS. This method can be used with other ELISA assays or procedures evaluating proteins.

Original languageEnglish
Article numbere57113
JournalJournal of Visualized Experiments
Volume2018
Issue number136
DOIs
Publication statusPublished - Jun 11 2018

Fingerprint

Tumor Biomarkers
Blood
Serum
Enzyme-Linked Immunosorbent Assay
Real-Time Polymerase Chain Reaction
Dry Ice
Assays
Single Nucleotide Polymorphism
Proteins
Thawing
Nucleotides
Polymorphism
Biological materials
DNA
Ice

Keywords

  • Blood
  • Cancer biomarkers
  • Cancer research
  • Dried blood spot
  • Dried serum spot
  • Issue 136
  • Sample storage
  • Serum/plasma

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

@article{62da76ade6824d3a80fe78487a237585,
title = "Dried blood and serum spots as a useful tool for sample storage to evaluate cancer biomarkers",
abstract = "Blood sample quality is crucial to ensure accurate downstream analyses such as real-time PCR or ELISA. Correct storage of biological materials is the starting point to achieve reproducible and reliable results. All samples should be treated in the same way from blood collection to storage. Depending on the analyses to be performed, whole blood and serum samples should be stored at-20 °C or-80 °C until use. Blood/serum samples should also be aliquoted to avoid multiple freeze-thawing. Another important issue is the sample conditions during shipment from one laboratory to another. If dry ice is not available or the shipment takes longer than a few days, alternative approaches are needed. One option is to use filter paper for blood collection. Here, we propose a method for blood and serum sample collection that takes advantage of dried blood spots (DBS) and dried serum spots (DSS). We developed the procedure to extract DNA from DBS for the downstream evaluation of some single nucleotide polymorphisms (SNPs) by real time PCR. We also optimized an ELISA assay starting from proteins eluted from DSS. This method can be used with other ELISA assays or procedures evaluating proteins.",
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author = "Laura Mercatali and Patrizia Serra and Giacomo Miserocchi and Chiara Spadazzi and Chiara Liverani and {De Vita}, Alessandro and Giorgia Marisi and Alberto Bongiovanni and Federica Recine and Akwilina Pangan and Nestory Masalu and Toni Ibrahim and Dino Amadori",
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T1 - Dried blood and serum spots as a useful tool for sample storage to evaluate cancer biomarkers

AU - Mercatali, Laura

AU - Serra, Patrizia

AU - Miserocchi, Giacomo

AU - Spadazzi, Chiara

AU - Liverani, Chiara

AU - De Vita, Alessandro

AU - Marisi, Giorgia

AU - Bongiovanni, Alberto

AU - Recine, Federica

AU - Pangan, Akwilina

AU - Masalu, Nestory

AU - Ibrahim, Toni

AU - Amadori, Dino

PY - 2018/6/11

Y1 - 2018/6/11

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