Droplet digital pcr analysis of liquid biopsy samples unveils the diagnostic role of hsa-mir-133a-3p and hsa-mir-375-3p in oral cancer

Salvatore Crimi, Luca Falzone, Giuseppe Gattuso, Caterina Maria Grillo, Saverio Candido, Alberto Bianchi, Massimo Libra

Research output: Contribution to journalArticlepeer-review

Abstract

Despite the availability of screening programs, oral cancer deaths are increasing due to the lack of diagnostic biomarkers leading to late diagnosis and a poor prognosis. Therefore, there is an urgent need to discover novel effective biomarkers for this tumor. On these bases, the aim of this study was to validate the diagnostic potential of microRNAs (miRNAs) through the analysis of liquid biopsy samples obtained from ten oral cancer patients and ten healthy controls. The expression of four selected miRNAs was evaluated by using droplet digital PCR (ddPCR) in a pilot cohort of ten oral cancer patients and ten healthy donors. Bioinformatics analyses were performed to assess the functional role of these miRNAs. The expression levels of the predicted down-regulated hsa-miR-133a-3p and hsa-miR-375-3p were significantly reduced in oral cancer patients compared to normal individuals while no significant results were obtained for the up-regulated hsa-miR-503-5p and hsa-miR-196a-5p. ROC analysis confirmed the high sensitivity and specificity of hsa-miR-375-3p and hsa-miR-133a-3p. Therefore, both miRNAs are significantly down-regulated in cancer patients and can be used as biomarkers for the early diagnosis of oral cancer. The analysis of circulating miRNAs in a larger series of patients is mandatory to confirm the results obtained in this pilot study.

Original languageEnglish
Article number379
Pages (from-to)1-14
Number of pages14
JournalBiology
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 2020

Keywords

  • Biomarkers
  • Cancer
  • DdPCR
  • Diagnosis
  • Epigenetics
  • MicroRNAs
  • Oral cancers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Droplet digital pcr analysis of liquid biopsy samples unveils the diagnostic role of hsa-mir-133a-3p and hsa-mir-375-3p in oral cancer'. Together they form a unique fingerprint.

Cite this