Droplet digital PCR quantification of miR-1290 as a circulating biomarker for pancreatic cancer

Francesca Tavano, Domenica Gioffreda, Maria R. Valvano, Orazio Palmieri, Matteo Tardio, Tiziana P. Latiano, Ada Piepoli, Evaristo Maiello, Felice Pirozzi, Angelo Andriulli

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Abstract

Droplet digital PCR was used to validate miR-1290 as circulating biomarker for pancreatic cancer (PC). The diagnostic performance of miR-1290 was evaluate in 167 PC patients and 267 healthy subjects at clinical risk of developing the disease (HS). MiR-1290 plasma levels were compared to CA 19-9 determinations, and the combination of the two biomarkers was also taken into account. Plasma levels of miR-1290 were higher in PC patients compared to HS (p = 2.55 × 10−16). A similar trend was observed for CA 19-9 determinations (p = 1.03 × 10−47). ROC curve analysis revealed that miR-1290 in combination with CA 19-9 was effective for discriminating between PC patients and HS (AUC = 0.956, 95% CI = 0.933–0.979) than the two biomarkers tested alone (miR-1290: AUC = 0.734, 0.678–0.789; CA 19-9: AUC = 0.914, 0.877–0.951). The discriminating ability was higher when only PC patients with low or slightly increased CA 19-9 levels were compared with HS. MiR-1290 concentrations were not able to differentiate between PC patients with single or multiple risk factors for developing PC. Our data suggest that the absolute quantification of circulating miR-1290 levels does not allow to select patients at clinical risk of PC for entry into a surveillance program, and underline the methodological challenges still existing in utilizing circulating miRNAs as new promising biomarkers for PC.

Original languageEnglish
Article number16389
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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