dsDNA-, nucleohistone- and DNASE I-reactive T lymphocytes in patients affected by systemic lupus erythematosus

Correlation with clinical disease activity

G. Filaci, I. Grasso, P. Contini, M. A. Imro, L. Lanza, M. Scudeletti, E. Rossi, F. Puppo, E. Damasio, F. Indiveri

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective. To demonstrate the involvement of T lymphocytes reactive to autoantigens in the pathogenesis of autoimmune diseases and to analyse their clinical relevance. Methods. The frequency of T cell clones reactive to double strand DNA (dsDNA), Nucleohistone (NH) complex and Dnase I was calculated for the peripheral blood mononuclear cells (PBMC) of 15 SLE patients and 9 healthy subjects by proliferation assay. Results. DsDNA- and NH-specific T cell clones were found in the majority of the patients analysed (frequency ranging from 2 to 50 clones/107 PBMC), while their absence or very low frequency (2 clones/107 PBMC) was observed in the control PBMC. Their frequency significantly correlated with decreased serum concentrations of C3 and C4 and with the systemic lupus erythematosus disease activity index (P = 0.03). A very low frequency of Dnase I-reactive T cell clones was observed in both SLE and healthy subjects. Conclusion. Our results suggest that dsDNA- and NH-reactive T lymphocytes may be involved in the pathogenesis of SLE and that their quantification in the peripheral blood of patients could be a useful tool to follow the clinical course of the disease.

Original languageEnglish
Pages (from-to)543-550
Number of pages8
JournalClinical and Experimental Rheumatology
Volume14
Issue number5
Publication statusPublished - Sep 1996

Fingerprint

Systemic Lupus Erythematosus
Clone Cells
Blood Cells
T-Lymphocytes
DNA
Deoxyribonuclease I
Healthy Volunteers
Autoimmune Diseases
Serum

Keywords

  • anti-DNA
  • cell-mediated immunity
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

dsDNA-, nucleohistone- and DNASE I-reactive T lymphocytes in patients affected by systemic lupus erythematosus : Correlation with clinical disease activity. / Filaci, G.; Grasso, I.; Contini, P.; Imro, M. A.; Lanza, L.; Scudeletti, M.; Rossi, E.; Puppo, F.; Damasio, E.; Indiveri, F.

In: Clinical and Experimental Rheumatology, Vol. 14, No. 5, 09.1996, p. 543-550.

Research output: Contribution to journalArticle

Filaci, G, Grasso, I, Contini, P, Imro, MA, Lanza, L, Scudeletti, M, Rossi, E, Puppo, F, Damasio, E & Indiveri, F 1996, 'dsDNA-, nucleohistone- and DNASE I-reactive T lymphocytes in patients affected by systemic lupus erythematosus: Correlation with clinical disease activity', Clinical and Experimental Rheumatology, vol. 14, no. 5, pp. 543-550.
Filaci, G. ; Grasso, I. ; Contini, P. ; Imro, M. A. ; Lanza, L. ; Scudeletti, M. ; Rossi, E. ; Puppo, F. ; Damasio, E. ; Indiveri, F. / dsDNA-, nucleohistone- and DNASE I-reactive T lymphocytes in patients affected by systemic lupus erythematosus : Correlation with clinical disease activity. In: Clinical and Experimental Rheumatology. 1996 ; Vol. 14, No. 5. pp. 543-550.
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