dsDNA-, nucleohistone- and DNASE I-reactive T lymphocytes in patients affected by systemic lupus erythematosus: Correlation with clinical disease activity

G. Filaci, I. Grasso, P. Contini, M. A. Imro, L. Lanza, M. Scudeletti, E. Rossi, F. Puppo, E. Damasio, F. Indiveri

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Abstract

Objective. To demonstrate the involvement of T lymphocytes reactive to autoantigens in the pathogenesis of autoimmune diseases and to analyse their clinical relevance. Methods. The frequency of T cell clones reactive to double strand DNA (dsDNA), Nucleohistone (NH) complex and Dnase I was calculated for the peripheral blood mononuclear cells (PBMC) of 15 SLE patients and 9 healthy subjects by proliferation assay. Results. DsDNA- and NH-specific T cell clones were found in the majority of the patients analysed (frequency ranging from 2 to 50 clones/107 PBMC), while their absence or very low frequency (2 clones/107 PBMC) was observed in the control PBMC. Their frequency significantly correlated with decreased serum concentrations of C3 and C4 and with the systemic lupus erythematosus disease activity index (P = 0.03). A very low frequency of Dnase I-reactive T cell clones was observed in both SLE and healthy subjects. Conclusion. Our results suggest that dsDNA- and NH-reactive T lymphocytes may be involved in the pathogenesis of SLE and that their quantification in the peripheral blood of patients could be a useful tool to follow the clinical course of the disease.

Original languageEnglish
Pages (from-to)543-550
Number of pages8
JournalClinical and Experimental Rheumatology
Volume14
Issue number5
Publication statusPublished - Sep 1996

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Systemic Lupus Erythematosus
Clone Cells
Blood Cells
T-Lymphocytes
DNA
Deoxyribonuclease I
Healthy Volunteers
Autoimmune Diseases
Serum

Keywords

  • anti-DNA
  • cell-mediated immunity
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

dsDNA-, nucleohistone- and DNASE I-reactive T lymphocytes in patients affected by systemic lupus erythematosus : Correlation with clinical disease activity. / Filaci, G.; Grasso, I.; Contini, P.; Imro, M. A.; Lanza, L.; Scudeletti, M.; Rossi, E.; Puppo, F.; Damasio, E.; Indiveri, F.

In: Clinical and Experimental Rheumatology, Vol. 14, No. 5, 09.1996, p. 543-550.

Research output: Contribution to journalArticle

Filaci, G, Grasso, I, Contini, P, Imro, MA, Lanza, L, Scudeletti, M, Rossi, E, Puppo, F, Damasio, E & Indiveri, F 1996, 'dsDNA-, nucleohistone- and DNASE I-reactive T lymphocytes in patients affected by systemic lupus erythematosus: Correlation with clinical disease activity', Clinical and Experimental Rheumatology, vol. 14, no. 5, pp. 543-550.
Filaci, G. ; Grasso, I. ; Contini, P. ; Imro, M. A. ; Lanza, L. ; Scudeletti, M. ; Rossi, E. ; Puppo, F. ; Damasio, E. ; Indiveri, F. / dsDNA-, nucleohistone- and DNASE I-reactive T lymphocytes in patients affected by systemic lupus erythematosus : Correlation with clinical disease activity. In: Clinical and Experimental Rheumatology. 1996 ; Vol. 14, No. 5. pp. 543-550.
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