Dual effect of Thymosin α 1 on human monocyte-derived dendritic cell in vitro stimulated with viral and bacterial toll-like receptor agonists

Elena Giacomini, Martina Severa, Melania Cruciani, Marilena P aola Etna, Fabiana Rizzo, Manuela Pardini, Carolina Scagnolari, Enrico Garaci, Eliana M arina Coccia

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVES: Thymosin α 1 (Tα1) recently gained interest as immune adjuvant for vaccines because of its ability to modulate the T-cell/dendritic cell (DC) axis and to improve antibody production. The objective of this study was to determine whether Tα1 would address in vitro the response of human primary monocyte-derived DC, crucial regulators of vaccine-induced immunity, upon exposure to different toll-like receptor (TLR) agonists or infection with viruses or bacteria.

METHODS: DC maturation and production of pro-inflammatory cytokines were analyzed.

RESULTS: Our data revealed a dual effect of Tα1 on DC biology upon viral or bacterial stimulation. Interestingly, Tα1 enhanced human leukocyte antigen (HLA)-I and II surface expression and secretion of IL-6, TNF-α and IL-8 when DCs were treated with viral TLR3 and TLR7/8 agonists. Similarly, in pandemic H1N1 influenza A-infected DCs, Tα1 raised the expression of maturation markers and type I and III Interferon (IFN). In contrast, following bacterial TLR2 and 4 stimulation, as well as upon Bacillus Calmette-Guerin infection, the presence of Tα1 in DC cultures drastically lowered the analyzed cellular parameters.

CONCLUSION: The knowledge that Tα1 pleiotropic effect might ameliorate anti-viral immune responses and, at the same time, dampen inflammation caused by bacterial infections could lay the groundwork for a more appropriate therapeutic application of this molecule.

Original languageEnglish
Pages (from-to)S59-S70
JournalExpert Opinion on Biological Therapy
Volume15
DOIs
Publication statusPublished - 2015

Keywords

  • dendritic cell
  • human
  • Thymosin α 1
  • toll-like receptor

ASJC Scopus subject areas

  • Medicine(all)

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