Abstract
Methylation of histone H3 at Lys 9 is causally linked to formation of heterochromatin and to long-term transcriptional repression. We report an unexpected pattern of H3 Lys 9 methylation occurring at a subset of inducible inflammatory genes. This pattern is characterized by relatively low constitutive levels of H3 Lys 9 methylation that are erased upon activation and restored concurrently with post-induction transcriptional repression. Changes in H3 Lys 9 methylation strongly correlate with RNA polymerase II recruitment and release. In particular, remethylation correlates with RNApolII release more strongly than does histone deacetylation. We propose that, by generating a window of time in which transcription is permitted, dynamic modulation of H3 Lys 9 methylation adds an additional regulatory level to transcriptional activation of tightly controlled inducible genes.
| Original language | English |
|---|---|
| Pages (from-to) | 2219-2224 |
| Number of pages | 6 |
| Journal | Genes and Development |
| Volume | 16 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - Sep 1 2002 |
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Keywords
- Histone code
- Histone methylation
- Histone methyltransferase
- Transcriptional repression
ASJC Scopus subject areas
- Developmental Biology
- Genetics
Cite this
Dynamic changes in histone H3 Lys 9 methylation occurring at tightly regulated inducible inflammatory genes. / Saccani, Simona; Natoli, Gioacchino.
In: Genes and Development, Vol. 16, No. 17, 01.09.2002, p. 2219-2224.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamic changes in histone H3 Lys 9 methylation occurring at tightly regulated inducible inflammatory genes
AU - Saccani, Simona
AU - Natoli, Gioacchino
PY - 2002/9/1
Y1 - 2002/9/1
N2 - Methylation of histone H3 at Lys 9 is causally linked to formation of heterochromatin and to long-term transcriptional repression. We report an unexpected pattern of H3 Lys 9 methylation occurring at a subset of inducible inflammatory genes. This pattern is characterized by relatively low constitutive levels of H3 Lys 9 methylation that are erased upon activation and restored concurrently with post-induction transcriptional repression. Changes in H3 Lys 9 methylation strongly correlate with RNA polymerase II recruitment and release. In particular, remethylation correlates with RNApolII release more strongly than does histone deacetylation. We propose that, by generating a window of time in which transcription is permitted, dynamic modulation of H3 Lys 9 methylation adds an additional regulatory level to transcriptional activation of tightly controlled inducible genes.
AB - Methylation of histone H3 at Lys 9 is causally linked to formation of heterochromatin and to long-term transcriptional repression. We report an unexpected pattern of H3 Lys 9 methylation occurring at a subset of inducible inflammatory genes. This pattern is characterized by relatively low constitutive levels of H3 Lys 9 methylation that are erased upon activation and restored concurrently with post-induction transcriptional repression. Changes in H3 Lys 9 methylation strongly correlate with RNA polymerase II recruitment and release. In particular, remethylation correlates with RNApolII release more strongly than does histone deacetylation. We propose that, by generating a window of time in which transcription is permitted, dynamic modulation of H3 Lys 9 methylation adds an additional regulatory level to transcriptional activation of tightly controlled inducible genes.
KW - Histone code
KW - Histone methylation
KW - Histone methyltransferase
KW - Transcriptional repression
UR - http://www.scopus.com/inward/record.url?scp=0036712454&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036712454&partnerID=8YFLogxK
U2 - 10.1101/gad.232502
DO - 10.1101/gad.232502
M3 - Article
C2 - 12208844
AN - SCOPUS:0036712454
VL - 16
SP - 2219
EP - 2224
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 17
ER -