Dynamic NAD(P)H post-synaptic autofluorescence signals for the assessment of mitochondrial function in a neurodegenerative disease: Monitoring the primary motor cortex of G93A mice, an amyotrophic lateral sclerosis model

Stefano Loizzo, Massimo Pieri, Alberto Ferri, Maria Teresa Carrì, Cristina Zona, Andrea Fortuna, Stefano Vella

Research output: Contribution to journalArticle

Abstract

Abnormal mitochondrial function was reported in patients and models for amyotrophic lateral sclerosis (ALS). It is therefore important to set up sensitive tools for the monitoring of active agents that enhance energy metabolism delay onset, and extend lifespan of transgenic G93A-SOD1 ALS mice. In this report, primary motor cortex slices from G93A mice at different stages of disease were studied, using NAD(P)H autofluorescence post-synaptic signals following ultraviolet stimuli, as a probe to evaluate mitochondrial function. We observed consistent age-related alterations of responses in G93A primary motor cortex slices versus controls. We conclude that NAD(P)H autofluorescence post-synaptic signal is a highly sensitive real-time technique to detect mitochondrial function failure in primary cortex from living tissues.

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalMitochondrion
Volume10
Issue number2
DOIs
Publication statusPublished - Mar 2010

Keywords

  • G93A
  • Glucose metabolism
  • Mitochondrial complex I activity
  • NADH:ubiquinone oxidoreductase
  • SOD1
  • Spectrophotometric assay

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine

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