TY - JOUR
T1 - Dysregulated miR-671-5p / CDR1-AS / CDR1 / VSNL1 axis is involved in glioblastoma multiforme
AU - Barbagallo, Davide
AU - Condorelli, Angelo
AU - Ragusa, Marco
AU - Salito, Loredana
AU - Sammito, Mariangela
AU - Banelli, Barbara
AU - Caltabiano, Rosario
AU - Barbagallo, Giuseppe
AU - Zappalà, Agata
AU - Battaglia, Rosalia
AU - Cirnigliaro, Matilde
AU - Lanzafame, Salvatore
AU - Vasquez, Enrico
AU - Parenti, Rosalba
AU - Cicirata, Federico
AU - Di Pietro, Cinzia
AU - Romani, Massimo
AU - Purrello, Michele
PY - 2016
Y1 - 2016
N2 - MiR-671-5p is encoded by a gene localized at 7q36.1, a region amplified in human glioblastoma multiforme (GBM), the most malignant brain cancer. To investigate whether expression of miR-671-5p were altered in GBM, we analyzed biopsies from a cohort of forty-five GBM patients and from five GBM cell lines. Our data show significant overexpression of miR-671-5p in both biopsies and cell lines. By exploiting specific miRNA mimics and inhibitors, we demonstrated that miR-671-5p overexpression significantly increases migration and to a less extent proliferation rates of GBM cells. Through a combined in silico and in vitro approach, we identified CDR1-AS, CDR1, VSNL1 as downstream miR-671-5p targets in GBM. Expression of these genes significantly decreased both in GBM biopsies and cell lines and negatively correlated with that of miR-671-5p. Based on our data, we propose that the axis miR- 671-5p / CDR1-AS / CDR1 / VSNL1 is functionally altered in GBM cells and is involved in the modification of their biopathological profile.
AB - MiR-671-5p is encoded by a gene localized at 7q36.1, a region amplified in human glioblastoma multiforme (GBM), the most malignant brain cancer. To investigate whether expression of miR-671-5p were altered in GBM, we analyzed biopsies from a cohort of forty-five GBM patients and from five GBM cell lines. Our data show significant overexpression of miR-671-5p in both biopsies and cell lines. By exploiting specific miRNA mimics and inhibitors, we demonstrated that miR-671-5p overexpression significantly increases migration and to a less extent proliferation rates of GBM cells. Through a combined in silico and in vitro approach, we identified CDR1-AS, CDR1, VSNL1 as downstream miR-671-5p targets in GBM. Expression of these genes significantly decreased both in GBM biopsies and cell lines and negatively correlated with that of miR-671-5p. Based on our data, we propose that the axis miR- 671-5p / CDR1-AS / CDR1 / VSNL1 is functionally altered in GBM cells and is involved in the modification of their biopathological profile.
KW - Cell networks
KW - Circular RNAs (circRNAs)
KW - Glioblastoma multiforme (GBM)
KW - MicroRNAs (miRNAs)
KW - Non coding RNAs (ncRNAs)
UR - http://www.scopus.com/inward/record.url?scp=84957990770&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84957990770&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.6621
DO - 10.18632/oncotarget.6621
M3 - Article
AN - SCOPUS:84957990770
VL - 7
SP - 4746
EP - 4759
JO - Oncotarget
JF - Oncotarget
SN - 1949-2553
IS - 4
ER -