Dysregulated miR-671-5p / CDR1-AS / CDR1 / VSNL1 axis is involved in glioblastoma multiforme

Davide Barbagallo, Angelo Condorelli, Marco Ragusa, Loredana Salito, Mariangela Sammito, Barbara Banelli, Rosario Caltabiano, Giuseppe Barbagallo, Agata Zappalà, Rosalia Battaglia, Matilde Cirnigliaro, Salvatore Lanzafame, Enrico Vasquez, Rosalba Parenti, Federico Cicirata, Cinzia Di Pietro, Massimo Romani, Michele Purrello

Research output: Contribution to journalArticlepeer-review

Abstract

MiR-671-5p is encoded by a gene localized at 7q36.1, a region amplified in human glioblastoma multiforme (GBM), the most malignant brain cancer. To investigate whether expression of miR-671-5p were altered in GBM, we analyzed biopsies from a cohort of forty-five GBM patients and from five GBM cell lines. Our data show significant overexpression of miR-671-5p in both biopsies and cell lines. By exploiting specific miRNA mimics and inhibitors, we demonstrated that miR-671-5p overexpression significantly increases migration and to a less extent proliferation rates of GBM cells. Through a combined in silico and in vitro approach, we identified CDR1-AS, CDR1, VSNL1 as downstream miR-671-5p targets in GBM. Expression of these genes significantly decreased both in GBM biopsies and cell lines and negatively correlated with that of miR-671-5p. Based on our data, we propose that the axis miR- 671-5p / CDR1-AS / CDR1 / VSNL1 is functionally altered in GBM cells and is involved in the modification of their biopathological profile.

Original languageEnglish
Pages (from-to)4746-4759
Number of pages14
JournalOncotarget
Volume7
Issue number4
DOIs
Publication statusPublished - 2016

Keywords

  • Cell networks
  • Circular RNAs (circRNAs)
  • Glioblastoma multiforme (GBM)
  • MicroRNAs (miRNAs)
  • Non coding RNAs (ncRNAs)

ASJC Scopus subject areas

  • Oncology

Fingerprint Dive into the research topics of 'Dysregulated miR-671-5p / CDR1-AS / CDR1 / VSNL1 axis is involved in glioblastoma multiforme'. Together they form a unique fingerprint.

Cite this