Dysregulation of MS risk genes and pathways at distinct stages of disease

Sundararajan Srinivasan, Marco Di Dario, Alessandra Russo, Ramesh Menon, Elena Brini, Marzia Romeo, Francesca Sangalli, Gloria Dalla Costa, Mariaemma Rodegher, Marta Radaelli, Lucia Moiola, Daniela Cantarella, Enzo Medico, Gianvito Martino, Roberto Furlan, Vittorio Martinelli, Giancarlo Comi, Cinthia Farina

Research output: Contribution to journalArticle

Abstract

Objective: To perform systematic transcriptomic analysis of multiple sclerosis (MS) risk genes in peripheral blood mononuclear cells (PBMCs) of subjects with distinct MS stages and describe the pathways characterized by dysregulated gene expressions. Methods: We monitored gene expression levels in PBMCs from 3 independent cohorts for a total of 297 cases (including clinically isolated syndromes (CIS), relapsing-remitting MS, primary and secondary progressive MS) and 96 healthy controls by distinct microarray platforms and quantitative PCR. Differential expression and pathway analyses for distinct MS stages were defined and validated by literature mining. Results: Genes located in the vicinity of MS risk variants displayed altered expression in peripheral blood at distinct stages of MS compared with the healthy population. The frequency of dysregulation was significantly higher than expected in CIS and progressive forms of MS. Pathway analysis for each MS stage-specific gene list showed that dysregulated genes contributed to pathogenic processes with scientific evidence in MS. Conclusions: Systematic gene expression analysis in PBMCs highlighted selective dysregulation of MS susceptibility genes playing a role in novel and well-known pathogenic pathways.

Original languageEnglish
Article numbere337
JournalNeurology: Neuroimmunology and NeuroInflammation
Volume4
Issue number3
DOIs
Publication statusPublished - Jan 1 2017

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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