(E)-2-Cyano-3-(5′-piperidin-1-yl-2,2′-bithien-5-yl)acrylic acid: A fluorescent probe for detecting prefibrillar oligomers

Francesco Attanasio, Carmela Bonaccorso, Francesco Bellia, Sebastiano Cataldo, Cosimo G. Fortuna, Giuseppe Musumarra, Bruno Pignataro, Enrico Rizzarelli

Research output: Contribution to journalArticle

Abstract

The synthesis of (E)-2-cyano-3-(5′-piperidin-1-yl-2,2′-bithien- 5-yl)acrylic acid, a novel amyloid aggregation fluorescent probe, is reported. This new probe is able to monitor soluble oligomeric aggregates after 24 h, at which time Thioflavin T emission, commonly used to monitor amyloid fibril formation, remains unchanged. Atomic force microscopy, native polyacrylamide gel electrophoresis, and dynamic light scattering confirm that the earlier stages of aggregation are prefibrillar oligomeric species not possessing the amyloid structure. This new molecular scaffold expands the toolbox of fluorescent probes for the identification of prefibrillar oligomers, which is needed in studies aimed at the early detection of the soluble toxic aggregates that characterize the so-called protein misfolding diseases. (E)-2-Cyano-3-(5′-piperidin-1- yl-2,2′-bithien-5-yl)acrylic acid (p-TTCNAc) can be conveniently used as a fluorescent probe to identify prefibrillar oligomers of hen egg white lysozyme not detected by Thioflavin-T (ThT).

Original languageEnglish
Pages (from-to)3635-3639
Number of pages5
JournalEuropean Journal of Organic Chemistry
Issue number18
DOIs
Publication statusPublished - Jun 2013

Keywords

  • Aggregation
  • Atomic force microscopy
  • Electrophoresis
  • Fluorescent probes
  • Heterocycles

ASJC Scopus subject areas

  • Organic Chemistry
  • Physical and Theoretical Chemistry

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