E-cadherin genetic screening and clinico-pathologic characteristics of early onset gastric cancer

Giovanni Corso, Corrado Pedrazzani, Hugo Pinheiro, Eduardo Fernandes, Daniele Marrelli, Andrea Rinnovati, Valeria Pascale, Raquel Seruca, Carla Oliveira, Franco Roviello

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: CDH1 germline alterations occur in about 40% of hereditary diffuse gastric cancer (HDGC) families. CDH1 germline mutations are also documented in few early onset diffuse gastric cancer patients (EODGC) without family history, but the real frequency in this setting in unknown. In these patients, the advanced stage at the time of diagnosis remains a clinical burden due to the poor long term survival. Methods: The entire coding region and exon flanking sequences of the CDH1 gene was analysed by direct sequencing in 21 EODGC patients aged ≤50 years. The potential deleterious nature for a new CDH1 missense variant was assessed by cell-cell aggregation and invasion assays. Somatic CDH1 mutation, loss of heterozigosity (LOH) and promoter hypermethylation was explored in the tumour from one CDH1 germline mutation carrier. Results: Two novel CDH1 germline variants were identified in 21 EODGC cases, c.670C>T and -63C>A. Functional analysis of the c.670C>T missense variant classified this mutation as non-pathogenic. The analysis of CDH1 somatic second hits failed to demonstrate E-cadherin structural and epigenetic alterations in the tumour sample. Conclusion: Data from the present work and a systematic review of the literature revealed that CDH1 germline mutations occurred in 7.2% of EOGC patients invariably with diffuse of mixed histology. From these, proved CDH1 mutation pathogenicity has been assigned only to 2.3% of the cases who were recurrently diagnosed before 35 years old. Germline CDH1 mutation remain the only germline genetic defect described in this type of patients and CDH1 mutation screening should be recommended for patients with these characteristics.

Original languageEnglish
Pages (from-to)631-639
Number of pages9
JournalEuropean Journal of Cancer
Volume47
Issue number4
DOIs
Publication statusPublished - Mar 2011

Keywords

  • Diffuse gastric cancer
  • E-cadherin
  • Early onset gastric cancer
  • Family history
  • Genetic screening
  • Germline mutations
  • Hereditary and sporadic cancer
  • Pathology
  • Promoter methylation
  • Second hit

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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