Abstract
RecA protein was found to catalyze the dissociation of the strands of a DNA substrate consisting of a 20-nucleotide primer annealed to circular single-stranded M13mp DNA. The strand separation reaction requires ATP hydrolysis and the presence of single-stranded DNA flanking the duplex DNA region to be unwound. RecA-catalyzed strand separation is effective only for very short duplexes, not exceeding 30 bp, and is not stimulated by single-stranded DNA-binding protein. These results are consistent with the ability of recA protein to disrupt regions of secondary structure in single-stranded DNA and to incorporate large non-homologies into heteroduplex DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 3025-3030 |
| Number of pages | 6 |
| Journal | EMBO Journal |
| Volume | 4 |
| Issue number | 11 |
| Publication status | Published - Nov 1985 |
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ASJC Scopus subject areas
- Cell Biology
- Genetics
Cite this
E. coli recA protein possesses a strand separating activity on short duplex DNAs. / Bianchi, M.; Riboli, B.; Magni, G.
In: EMBO Journal, Vol. 4, No. 11, 11.1985, p. 3025-3030.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - E. coli recA protein possesses a strand separating activity on short duplex DNAs.
AU - Bianchi, M.
AU - Riboli, B.
AU - Magni, G.
PY - 1985/11
Y1 - 1985/11
N2 - RecA protein was found to catalyze the dissociation of the strands of a DNA substrate consisting of a 20-nucleotide primer annealed to circular single-stranded M13mp DNA. The strand separation reaction requires ATP hydrolysis and the presence of single-stranded DNA flanking the duplex DNA region to be unwound. RecA-catalyzed strand separation is effective only for very short duplexes, not exceeding 30 bp, and is not stimulated by single-stranded DNA-binding protein. These results are consistent with the ability of recA protein to disrupt regions of secondary structure in single-stranded DNA and to incorporate large non-homologies into heteroduplex DNA.
AB - RecA protein was found to catalyze the dissociation of the strands of a DNA substrate consisting of a 20-nucleotide primer annealed to circular single-stranded M13mp DNA. The strand separation reaction requires ATP hydrolysis and the presence of single-stranded DNA flanking the duplex DNA region to be unwound. RecA-catalyzed strand separation is effective only for very short duplexes, not exceeding 30 bp, and is not stimulated by single-stranded DNA-binding protein. These results are consistent with the ability of recA protein to disrupt regions of secondary structure in single-stranded DNA and to incorporate large non-homologies into heteroduplex DNA.
UR - http://www.scopus.com/inward/record.url?scp=0022160321&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022160321&partnerID=8YFLogxK
M3 - Article
C2 - 3905387
AN - SCOPUS:0022160321
VL - 4
SP - 3025
EP - 3030
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 11
ER -