E-FABP induces differentiation in normal human keratinocytes and modulates the differentiation process in psoriatic keratinocytes in vitro

Katiuscia Dallaglio, Alessandra Marconi, Francesca Truzzi, Roberta Lotti, Elisabetta Palazzo, Tiziana Petrachi, Annalisa Saltari, Maurizio Coppini, Carlo Pincelli

Research output: Contribution to journalArticlepeer-review

Abstract

Epidermal fatty acid-binding protein (E-FABP) is a lipid carrier, originally discovered in human epidermis. We show that E-FABP is almost exclusively expressed in postmitotic (PM) keratinocytes, corresponding to its localization in the highest suprabasal layers, while it is barely expressed in keratinocyte stem cells (KSC) and transit amplifying (TA) keratinocytes. Transfection of normal human keratinocytes with recombinant (r) E-FABP induces overexpression of K10 and involucrin. On the other hand, E-FABP inhibition by siRNA downregulates K10 and involucrin expression in normal keratinocytes through NF-κB and JNK signalling pathways. E-FABP is highly expressed in psoriatic epidermis, and it is mainly localized in stratum spinosum. Psoriatic PM keratinocytes overexpress E-FABP as compared to the same population in normal epidermis. E-FABP inhibition in psoriatic keratinocytes markedly reduces differentiation, while it upregulates psoriatic markers such as survivin and K16. However, under high-calcium conditions, E-FABP silencing downregulates K10 and involucrin, while survivin and K16 expression is completely abolished. These data strongly indicate that E-FABP plays an important role in keratinocyte differentiation. Moreover, E-FABP modulates differentiation in psoriatic keratinocytes.

Original languageEnglish
Pages (from-to)255-261
Number of pages7
JournalExperimental Dermatology
Volume22
Issue number4
DOIs
Publication statusPublished - Apr 2013

Keywords

  • E-FABP
  • Keratinocytes
  • Psoriasis
  • Skin
  • Subpopulations

ASJC Scopus subject areas

  • Dermatology
  • Molecular Biology
  • Biochemistry

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