E1 detection as prognosticates in human papillomavirus-positive head and neck cancers

Daniela Vivenza, Cristiana Lo Nigro, Nerina Denaro, Mirella Fortunato, Martino Monteverde, Federica Tonissi, Laura Lattanzio, Valentina Astesana, Annunziata Gloghini, Chiara C. Volpi, Elvio Russi, Marco C. Merlano

Research output: Contribution to journalArticlepeer-review


Purpose: HPV-related locally advanced head and neck cancers (LA-HNCs) show a good prognosis. This study aimed to investigate the HPV prevalence in LA-HNCs and compare the prognostic value of E1, E6 and L1 genomic viral fragments and p16, individually and in combination, in order to And the best prognosticator in terms of overall survival (OS) and progression-free survival (PFS). Patients and methods: HPV16 was searched in 255 LA-HNC formalin-fixed paraffin-embedded tumor tissues, 89 oropharyngeal cancers (OPCs), and 166 non-OPCs by DNA-PCR with 3 primer pairs. p16 was analyzed by immunohistochemistry in 235 patients. Results: The prevalence of positive samples decreased constantly from E6 to L1 and E1 in both OPCs and non-OPCs. Each LA-HNC patient highlighted variable positivity for each fragment. OPCs showed a higher prevalence of positive samples compared to non-OPCs. Positive coexistence of all the fragments was more common in OPCs (31.5%) than non-OPCs (4.2%), and E1 detection was always associated with E6 and L1. E1-positive OPCs showed improved OS (p = 0.012) and PFS (p = 0.036), while L1- or E6-positive ones did not. p16-positive patients were more prevalent in the OPC (29.8%) than the non-OPC group (7.3%) (p

Original languageEnglish
Pages (from-to)e163-e172
JournalInternational Journal of Biological Markers
Issue number2
Publication statusPublished - Apr 1 2016


  • Human papillomavirus
  • Oropharyngeal cancer
  • p16-IHC
  • Prognostic value

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine


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