E1 detection as prognosticates in human papillomavirus-positive head and neck cancers

Daniela Vivenza; Cristiana Lo Nigro; Nerina Denaro; Mirella Fortunato; Martino Monteverde; Federica Tonissi; Laura Lattanzio; Valentina Astesana; Annunziata Gloghini; Chiara C. Volpi; Elvio Russi; Marco C. Merlano

doi:10.5301/jbm.5000193

E1 detection as prognosticates in human papillomavirus-positive head and neck cancers

Daniela Vivenza, Cristiana Lo Nigro, Nerina Denaro, Mirella Fortunato, Martino Monteverde, Federica Tonissi, Laura Lattanzio, Valentina Astesana, Annunziata Gloghini, Chiara C. Volpi, Elvio Russi, Marco C. Merlano

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose: HPV-related locally advanced head and neck cancers (LA-HNCs) show a good prognosis. This study aimed to investigate the HPV prevalence in LA-HNCs and compare the prognostic value of E1, E6 and L1 genomic viral fragments and p16, individually and in combination, in order to And the best prognosticator in terms of overall survival (OS) and progression-free survival (PFS). Patients and methods: HPV16 was searched in 255 LA-HNC formalin-fixed paraffin-embedded tumor tissues, 89 oropharyngeal cancers (OPCs), and 166 non-OPCs by DNA-PCR with 3 primer pairs. p16 was analyzed by immunohistochemistry in 235 patients. Results: The prevalence of positive samples decreased constantly from E6 to L1 and E1 in both OPCs and non-OPCs. Each LA-HNC patient highlighted variable positivity for each fragment. OPCs showed a higher prevalence of positive samples compared to non-OPCs. Positive coexistence of all the fragments was more common in OPCs (31.5%) than non-OPCs (4.2%), and E1 detection was always associated with E6 and L1. E1-positive OPCs showed improved OS (p = 0.012) and PFS (p = 0.036), while L1- or E6-positive ones did not. p16-positive patients were more prevalent in the OPC (29.8%) than the non-OPC group (7.3%) (p

Original language	English
Pages (from-to)	e163-e172
Journal	International Journal of Biological Markers
Volume	31
Issue number	2
DOIs	https://doi.org/10.5301/jbm.5000193
Publication status	Published - Apr 1 2016

Keywords

DNA-PCR
Human papillomavirus
Oropharyngeal cancer
p16-IHC
Prognostic value

ASJC Scopus subject areas

Clinical Biochemistry
Cancer Research
Oncology
Pathology and Forensic Medicine

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E1 detection as prognosticates in human papillomavirus-positive head and neck cancers. / Vivenza, Daniela; Lo Nigro, Cristiana; Denaro, Nerina; Fortunato, Mirella; Monteverde, Martino; Tonissi, Federica; Lattanzio, Laura; Astesana, Valentina; Gloghini, Annunziata ; Volpi, Chiara C.; Russi, Elvio; Merlano, Marco C.

In: International Journal of Biological Markers, Vol. 31, No. 2, 01.04.2016, p. e163-e172.

Research output: Contribution to journal › Article › peer-review

Vivenza, Daniela ; Lo Nigro, Cristiana ; Denaro, Nerina ; Fortunato, Mirella ; Monteverde, Martino ; Tonissi, Federica ; Lattanzio, Laura ; Astesana, Valentina ; Gloghini, Annunziata ; Volpi, Chiara C. ; Russi, Elvio ; Merlano, Marco C. / E1 detection as prognosticates in human papillomavirus-positive head and neck cancers. In: International Journal of Biological Markers. 2016 ; Vol. 31, No. 2. pp. e163-e172.

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abstract = "Purpose: HPV-related locally advanced head and neck cancers (LA-HNCs) show a good prognosis. This study aimed to investigate the HPV prevalence in LA-HNCs and compare the prognostic value of E1, E6 and L1 genomic viral fragments and p16, individually and in combination, in order to And the best prognosticator in terms of overall survival (OS) and progression-free survival (PFS). Patients and methods: HPV16 was searched in 255 LA-HNC formalin-fixed paraffin-embedded tumor tissues, 89 oropharyngeal cancers (OPCs), and 166 non-OPCs by DNA-PCR with 3 primer pairs. p16 was analyzed by immunohistochemistry in 235 patients. Results: The prevalence of positive samples decreased constantly from E6 to L1 and E1 in both OPCs and non-OPCs. Each LA-HNC patient highlighted variable positivity for each fragment. OPCs showed a higher prevalence of positive samples compared to non-OPCs. Positive coexistence of all the fragments was more common in OPCs (31.5%) than non-OPCs (4.2%), and E1 detection was always associated with E6 and L1. E1-positive OPCs showed improved OS (p = 0.012) and PFS (p = 0.036), while L1- or E6-positive ones did not. p16-positive patients were more prevalent in the OPC (29.8%) than the non-OPC group (7.3%) (p",

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AU - Denaro, Nerina

AU - Fortunato, Mirella

AU - Monteverde, Martino

AU - Tonissi, Federica

AU - Lattanzio, Laura

AU - Astesana, Valentina

AU - Gloghini, Annunziata

AU - Volpi, Chiara C.

AU - Russi, Elvio

AU - Merlano, Marco C.

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