Abstract
Objective. We retrospectively investigated a cohort of very low birthweight infants (VLBWI) by comparing two methods for assessment of albuminuria: nephelometry (standard) and high performance liquid chromatography (HPLC), measuring urinary immunoreactive and non-immunoreactive albumin to evaluate if the latter is more sensitive in identifying childhood onset of albuminuria and hypertension in VLBWI individuals. Methods. Spot urine samples of a total of 109 subjects (5.3±2.2 years old) who were VLBWI were investigated by HPLC and nephelometry. Twenty-eight subjects were small for gestational age (SGA) and 81 were appropriate for gestational age (AGA). Blood Pressure (BP) was also recorded. Results. Twelve children (11%) with albuminuria 20 mg/g Cr by nephelometry versus 48 (44.5%) by HPLC (p <0.001) were identified. The mean±SD of values in the range of normoalbuminuria was 2.9±5.81 by nephelometry versus 22.6±14 for HPLC (p <0.001). The values were not statistically different in the two birthweight categories (90th percentile) versus normal BP children. Conclusions. Microalbuminuria by HPLC was more marked in VLBWI than in adults and the difference between values using the two methods was significative.
| Original language | English |
|---|---|
| Pages (from-to) | 83-88 |
| Number of pages | 6 |
| Journal | Journal of Maternal-Fetal and Neonatal Medicine |
| Volume | 22 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2009 |
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Keywords
- Hypertension in children
- Microalbuminuria
- Newborns
- Urinary biomarkers
- Very low birthweight
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Pediatrics, Perinatology, and Child Health
Cite this
Early detection of microalbuminuria and hypertension in children of very low birthweight. / Melania, Puddu; Francesca, Podda Maria; Michele, Mussap; Roberto, Tumbarello; Vassilios, Fanos.
In: Journal of Maternal-Fetal and Neonatal Medicine, Vol. 22, No. 2, 02.2009, p. 83-88.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Early detection of microalbuminuria and hypertension in children of very low birthweight
AU - Melania, Puddu
AU - Francesca, Podda Maria
AU - Michele, Mussap
AU - Roberto, Tumbarello
AU - Vassilios, Fanos
PY - 2009/2
Y1 - 2009/2
N2 - Objective. We retrospectively investigated a cohort of very low birthweight infants (VLBWI) by comparing two methods for assessment of albuminuria: nephelometry (standard) and high performance liquid chromatography (HPLC), measuring urinary immunoreactive and non-immunoreactive albumin to evaluate if the latter is more sensitive in identifying childhood onset of albuminuria and hypertension in VLBWI individuals. Methods. Spot urine samples of a total of 109 subjects (5.3±2.2 years old) who were VLBWI were investigated by HPLC and nephelometry. Twenty-eight subjects were small for gestational age (SGA) and 81 were appropriate for gestational age (AGA). Blood Pressure (BP) was also recorded. Results. Twelve children (11%) with albuminuria 20 mg/g Cr by nephelometry versus 48 (44.5%) by HPLC (p <0.001) were identified. The mean±SD of values in the range of normoalbuminuria was 2.9±5.81 by nephelometry versus 22.6±14 for HPLC (p <0.001). The values were not statistically different in the two birthweight categories (90th percentile) versus normal BP children. Conclusions. Microalbuminuria by HPLC was more marked in VLBWI than in adults and the difference between values using the two methods was significative.
AB - Objective. We retrospectively investigated a cohort of very low birthweight infants (VLBWI) by comparing two methods for assessment of albuminuria: nephelometry (standard) and high performance liquid chromatography (HPLC), measuring urinary immunoreactive and non-immunoreactive albumin to evaluate if the latter is more sensitive in identifying childhood onset of albuminuria and hypertension in VLBWI individuals. Methods. Spot urine samples of a total of 109 subjects (5.3±2.2 years old) who were VLBWI were investigated by HPLC and nephelometry. Twenty-eight subjects were small for gestational age (SGA) and 81 were appropriate for gestational age (AGA). Blood Pressure (BP) was also recorded. Results. Twelve children (11%) with albuminuria 20 mg/g Cr by nephelometry versus 48 (44.5%) by HPLC (p <0.001) were identified. The mean±SD of values in the range of normoalbuminuria was 2.9±5.81 by nephelometry versus 22.6±14 for HPLC (p <0.001). The values were not statistically different in the two birthweight categories (90th percentile) versus normal BP children. Conclusions. Microalbuminuria by HPLC was more marked in VLBWI than in adults and the difference between values using the two methods was significative.
KW - Hypertension in children
KW - Microalbuminuria
KW - Newborns
KW - Urinary biomarkers
KW - Very low birthweight
UR - http://www.scopus.com/inward/record.url?scp=67249134000&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67249134000&partnerID=8YFLogxK
U2 - 10.1080/14767050802360809
DO - 10.1080/14767050802360809
M3 - Article
VL - 22
SP - 83
EP - 88
JO - Journal of Maternal-Fetal and Neonatal Medicine
JF - Journal of Maternal-Fetal and Neonatal Medicine
SN - 1476-7058
IS - 2
ER -