Early detection of Mycobacterium tuberculosis in BACTEC cultures by ligase chain reaction

E. Tortoli, F. Lavinia, M. T. Simonetti

Research output: Contribution to journalArticle

Abstract

The LCx Mycobacterium tuberculosis ligase chain reaction system (Abbott Diagnostic Division, Abbott Park, III.) was used to detect M. tuberculosis in 150 consecutive BACTEC vials on the day on which a positive growth index (GI) was recorded. By LCx, M. tuberculosis DNA was detected in BACTEC vials on average 2.6 days before the presence of acid-fast bacilli could be confirmed by microscopic examination. A total of 106 of 108 M. tuberculosis isolates were detected without centrifugation from bottles presenting very low GIs (average, 70; median, 33). No false-positive result was obtained from nontuberculous mycobacteria or from isolates with contaminants.

Original languageEnglish
Pages (from-to)2791-2792
Number of pages2
JournalJournal of Clinical Microbiology
Volume36
Issue number9
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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