Early effects of extracorporeal shock wave treatment on osteoblast-like cells: A comparative study between electromagnetic and electrohydraulic devices

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Abstract

BACKGROUND: Extracorporeal shockwave therapy (ESWT) has been increasingly applied to treat orthopedic and musculoskeletal pathologies. ESWT involves mechanical perturbations that, as with other physical therapies, can result in mechanical stimuli to a large number of cells, including bone cells. The aim of this study was to evaluate the effects of shock waves on osteoblast-like cells (MG63) when using two different generators of shock waves (electrohydraulic and electromagnetic devices), in terms of cell damage, cell viability, osteogenic phenotype expression, and cytokine production. METHODS: MG63 cells were suspended in 1.5 mL screw-cap cryotubes (1 × 10 cells/mL), containing phosphate buffer solution (PBS), which were maintained at 37°C during all the experimental times. Two levels of energy flux density (EFD) were evaluated for each device: 0.15 to 0.18 mJ/mm and 0.40 mJ/mm. Cells were then cultivated for 72 hours starting from a concentration of 1 × 10 cells/mL, and biological activity and viability were evaluated 24 and 72 hours after treatment. RESULTS: The results obtained demonstrate that the factors most affecting osteoblast activity involve both the device and the level of EFD selected, and they must be considered all together. CONCLUSIONS: The use of the electromagnetic device and a level of EFD lower than 0.40 mJ/mm would appear to induce fewer immediate cytodestructive effects and better stimulate subsequent proliferation and the synthetic activity of MG63.

Original languageEnglish
Pages (from-to)1198-1206
Number of pages9
JournalThe Journal of trauma
Volume61
Issue number5
DOIs
Publication statusPublished - Nov 2006

Keywords

  • Electrohydraulic
  • Electromagnetic
  • Extracorporeal shock wave treatment
  • Osteoblast-like cells

ASJC Scopus subject areas

  • Surgery

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