Early effects of portal flow modulation after extended liver resection in rat

Stefano Di Domenico, Gregorio Santori, Nicola Traverso, Emanuela Balbis, Annalisa Furfaro, Federica Grillo, Raffaella Gentile, Bruna Bocca, Maximiliano Gelli, Enzo Andorno, Ahmed Dahame, Damiano Cottalasso, Umberto Valente

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: The incidence of small-for-size-liver-syndrome after liver transplantation and extended liver resection may be reduced by portal flow modulation. However, many aspects of the small-for-size-liver-syndrome pathogenesis are still unclear. In this experimental study we evaluated the early effects of portal flow modulation after 80% hepatic resection in rats. Materials and methods: Rats were randomised in: sham operation (G1), conventional hepatic resection (G2), splenectomy and hepatic resection (G3), splenic transposition followed by hepatic resection after three weeks (G4). Six hours after operation, oxygen saturation of hepatic vein blood, glutathione, and standard liver markers were measured from hepatic venous blood. Glutathione measurement and histopatological examination were performed in the remnant liver. Results: Total bilirubin and liver glutathione did not show differences between groups. Aspartate aminotransferase and alanine aminotransferase significantly increased in G2-G4 groups. Blood glutathione and oxygen saturation of hepatic vein blood were lower in G2 than in other groups. A gradient of micro-vesicular degeneration was more severe in G2 compared with G3 and G4. Apoptosis, hemorrhagic necrosis, mitochondrial damage and leucocyte adhesion were evident in G2. Conclusion: The portal flow modulation induced by splenectomy or splenic transposition was effective in limiting early damage after extended liver resection.

Original languageEnglish
Pages (from-to)814-822
Number of pages9
JournalDigestive and Liver Disease
Volume43
Issue number10
DOIs
Publication statusPublished - Oct 2011

Keywords

  • Glutathione
  • Liver resection
  • Portal flow
  • Small for size liver syndrome

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

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