Early events in thymocyte activation. II. Changes in nonhistone chromatin proteins induced by a thymus-dependent human serum factor

A. Facchimi, G. C. Astaldi, L. Cocco, P. Wisermans, G. A. Manzoli, A. Astaldi

Research output: Contribution to journalArticlepeer-review

Abstract

Previously, it has been shown that a human thymus-dependent serum factor (SF), isolated from peripheral blood and acting on precursors of mature T lymphocytes, induces an increase in the synthesis of cyclic AMP and proteins in thymocytes. The authors have now investigated the action of SF on the incorporation of 3H-leucine and 32P-orthophosphate into nuclear proteins of thymocytes after 15 to 240 min of culture. SF induced a rapid increase in the synthesis of phosphorylation of nuclear proteins, especially in the phosphorylated nonhistone chromatin proteins (P-NHCP). Electrophoretic patterns in polyacrylamide gels of the P-NHCP fractions, extracted from the chromatin of the stimulated cells, showed that proteins with m.w. higher than 50 x 103 were synthesized to a larger extent as compared with unstimulated cells. These data suggest that SF acts specifically on the synthesis of P-NHCP and may in this way control DNA-template activity.

Original languageEnglish
Pages (from-to)1577-1585
Number of pages9
JournalJournal of Immunology
Volume123
Issue number4
Publication statusPublished - 1979

ASJC Scopus subject areas

  • Immunology

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