Early events of experimental exposure to amorphous and crystalline silica in the rat: time course of surfactant protein D.

M. Barbaro, G. Cutroneo, C. Costa, S. Sciorio, F. Trimarchi, A. Favaloro, C. Fenga, L. Barbaro Martino, G. Spatari, C. Abbate, P. Bramanti

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Abstract

Pneumoconioses determined by chronic inhalation of different kinds of silica present with peculiar clinical and histopathological features. Silicosis, caused by crystalline silica, is characterized by typical fibrous parenchymal nodules. Less defined are pneumoconioses due to amorphous silica. Aim of current experimental research on silicosis is to investigate the early events that lead to nodular fibrosis of the lung. A secretory component of the pulmonary environment, surfactant, seems to be involved in silica toxicity; surfactant protein D is a protein constituent, apparently involved in the homeostasis of the phospholipid component. We studied the behaviour of SP-D 2, 12 and 24 hours after treatment with 200 mg/kg crystalline silica or pumice powder suspended in 400 microl/kg saline solution and instiled intratracheally to rats. Both immunohistochemical localization and immunoblotting quantification demonstrated a sensible increase in intracellular SP-D, localized in alveolar type II cells and some bronchiolar epithelial cells, 2 hours after treatment. Increment appears less marked 12 hours after administration, reaching again levels comparable to control at 24 hours. The behaviour of SP-D after pumice instilation is similar, but with a significantly minor increment at 2 hours. These results indicate crystalline silica as responsible for a stronger acute injury of pulmonary tissue.

Original languageEnglish
Pages (from-to)243-256
Number of pages14
JournalItalian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia
Volume107
Issue number4
Publication statusPublished - Oct 2002

ASJC Scopus subject areas

  • Medicine(all)

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    Barbaro, M., Cutroneo, G., Costa, C., Sciorio, S., Trimarchi, F., Favaloro, A., Fenga, C., Barbaro Martino, L., Spatari, G., Abbate, C., & Bramanti, P. (2002). Early events of experimental exposure to amorphous and crystalline silica in the rat: time course of surfactant protein D. Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia, 107(4), 243-256.