Early Exposure to a High-Fat Diet Impacts on Hippocampal Plasticity

Implication of Microglia-Derived Exosome-like Extracellular Vesicles

Angeles Vinuesa, Melisa Bentivegna, Gastón Calfa, Fabia Filipello, Carlos Pomilio, María Marta Bonaventura, Victoria Lux-Lantos, María Eugenia Matzkin, Amal Gregosa, Jessica Presa, Michela Matteoli, Juan Beauquis, Flavia Saravia

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Adolescence is a transitional period from childhood to adulthood characterized by puberty and brain maturation involving behavioral changes and environmental vulnerability. Diet is one of the factors affecting brain health, potentially leading to long-lasting effects. Hence, we studied the impact of early exposure (P21-60) to a high-fat diet (HFD) on mouse hippocampus, analyzing inflammation, adult neurogenesis, dendritic spine plasticity, and spatial memory. Glycemia and seric pro-inflammatory IL1β were higher in HFD mice without differences on body weight. In the HFD hippocampus, neuroinflammation was evidenced by Iba1+ cells reactivity together with a higher expression of TNFα and IL1β while the neurogenic capability in the dentate gyrus was strongly reduced. We found a predominance of immature Dil-labeled dendritic spines from CA1 neurons along with diminished levels of the scaffold protein Shank2, suggesting a defective connectivity. Moreover, the HFD group exhibited spatial memory alterations. To elucidate whether microglia could be mediating HFD-associated neuronal changes, the lipotoxic context was emulated by incubating primary microglia with palmitate, a saturated fatty acid present in HFD. Palmitate induced a pro-inflammatory profile as shown by secreted cytokine levels. The isolated exosome fraction from palmitate-stimulated microglia induced an immature dendritic spine phenotype in primary GFP+ hippocampal neurons, in line with the in vivo findings. These results provide novel data concerning microglia to neuron communication and highlight that fat excess during a short and early period of life could negatively impact on cognition and synaptic plasticity in a neuroinflammatory context, where microglia-derived exosomes could be implicated. [Figure not available: see fulltext.].

Original languageEnglish
JournalMolecular Neurobiology
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Exosomes
Microglia
High Fat Diet
Dendritic Spines
Palmitates
Neurons
Hippocampus
Neuronal Plasticity
Neurogenesis
Dentate Gyrus
Brain
Puberty
Cognition
Extracellular Vesicles
Fatty Acids
Fats
Communication
Body Weight
Cytokines
Diet

Keywords

  • Adolescence
  • Dendritic spines
  • Exosomes
  • High-fat diet
  • Hippocampus
  • Microglia

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Early Exposure to a High-Fat Diet Impacts on Hippocampal Plasticity : Implication of Microglia-Derived Exosome-like Extracellular Vesicles. / Vinuesa, Angeles; Bentivegna, Melisa; Calfa, Gastón; Filipello, Fabia; Pomilio, Carlos; Bonaventura, María Marta; Lux-Lantos, Victoria; Matzkin, María Eugenia; Gregosa, Amal; Presa, Jessica; Matteoli, Michela; Beauquis, Juan; Saravia, Flavia.

In: Molecular Neurobiology, 01.01.2018.

Research output: Contribution to journalArticle

Vinuesa, A, Bentivegna, M, Calfa, G, Filipello, F, Pomilio, C, Bonaventura, MM, Lux-Lantos, V, Matzkin, ME, Gregosa, A, Presa, J, Matteoli, M, Beauquis, J & Saravia, F 2018, 'Early Exposure to a High-Fat Diet Impacts on Hippocampal Plasticity: Implication of Microglia-Derived Exosome-like Extracellular Vesicles', Molecular Neurobiology. https://doi.org/10.1007/s12035-018-1435-8
Vinuesa, Angeles ; Bentivegna, Melisa ; Calfa, Gastón ; Filipello, Fabia ; Pomilio, Carlos ; Bonaventura, María Marta ; Lux-Lantos, Victoria ; Matzkin, María Eugenia ; Gregosa, Amal ; Presa, Jessica ; Matteoli, Michela ; Beauquis, Juan ; Saravia, Flavia. / Early Exposure to a High-Fat Diet Impacts on Hippocampal Plasticity : Implication of Microglia-Derived Exosome-like Extracellular Vesicles. In: Molecular Neurobiology. 2018.
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