Early gene regulation by nerve growth factor in PC12 cells

Induction of an interferon-related gene

F. Tirone, E. M. Shooter

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Nerve growth factor (NGF) induces the chromaffin cell line PC12 to differentiate into cells with many of the properties of sympathetic neurons. We investigated the early differentiative phase and identified a gene, PC4, rapidly and transiently induced by NGF in PC12 cells. PC4 cDNA is homologous to the partial sequence of a putative mouse β-interferon and encodes a protein related to a lymphokine, the rat γ-interferon protein. Nonetheless, PC4 appears devoid of antiviral activity. PC4 is expressed in proliferating and differentiating tissues, such as amnion, placenta, and brain at embryonic day 13.5. The relationship of PC4 to interferons and lymphokines suggests that it could play a role in regulating gene activity in the pathways induced by NGF.

Original languageEnglish
Pages (from-to)2088-2092
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number6
Publication statusPublished - 1989

Fingerprint

PC12 Cells
Nerve Growth Factor
Interferons
Lymphokines
Genes
Chromaffin Cells
Amnion
Placenta
Antiviral Agents
Proteins
Complementary DNA
Neurons
Cell Line
Brain

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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AB - Nerve growth factor (NGF) induces the chromaffin cell line PC12 to differentiate into cells with many of the properties of sympathetic neurons. We investigated the early differentiative phase and identified a gene, PC4, rapidly and transiently induced by NGF in PC12 cells. PC4 cDNA is homologous to the partial sequence of a putative mouse β-interferon and encodes a protein related to a lymphokine, the rat γ-interferon protein. Nonetheless, PC4 appears devoid of antiviral activity. PC4 is expressed in proliferating and differentiating tissues, such as amnion, placenta, and brain at embryonic day 13.5. The relationship of PC4 to interferons and lymphokines suggests that it could play a role in regulating gene activity in the pathways induced by NGF.

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